糖尿病大鼠肝脏组织中维生素D受体表达与血清25-羟维生素D相关性研究

发布时间：2019-04-24 05:28

【摘要】：目的检测T2DM大鼠肝脏中维生素D受体(VDR)mRNA及蛋白表达,探讨T2DM大鼠肝脏VDR表达及血清25-羟维生素D[25(OH)D]与代谢指标的相关性。方法取60只健康雄性Wistar大鼠,随机分成T2DM组(n=30)和正常对照(NC)组(n=30)。T2DM组予高脂高胆固醇饲料喂养4周后,禁食16 h,按30 mg/kg单次腹腔注射STZ,72 h后尾静脉采血测血糖,以血糖浓度16.7mmol/L作为糖尿病模型造模成功的标准。ELISA测定血清25(OH)D水平,RT-PCR和Western blot分别检测两组肝脏组织中VDR mRNA及蛋白表达。Spearman相关分析T2DM组血清25(OH)D与FPG、F1ns、FFA、TG、TC、HOMA-IR的关系。结果与NC组比较,T2DM组肝脏中VDR mRNA及蛋白表达增高[(2.15±0.25)vs(8.26±2.23),(0.317±0.085)vs(0.542±0.135)],血清25(OH)D水平降低[(43.6±12.3)vs(21.3±6.7)ng/ml],血清FFA、TG、TC、LDL-C、FIns、HOMA-IR增高(P0.05)。相关性分析显示,血清25(OH)D与FFA、TG、FIns、HOMA-IR呈负相关,与HDL-C呈正相关(P0.05)。结论 T2DM大鼠肝脏组织中VDR mRNA及蛋白表达增加,而血清25(OH)D降低。血清25(OH)D降低可能会反馈性促进肝脏VDR表达,并可能进一步导致肝脏IR及糖脂代谢异常。
[Abstract]:Aim to detect the expression of vitamin D receptor (VDR) (VDR) mRNA and protein in the liver of T2DM rats, and to explore the relationship between the expression of VDR and serum 25-hydroxyvitamin D [25 (OH) D] in the liver of T2DM rats. Methods Sixty healthy male Wistar rats were randomly divided into two groups: T2DM group (n = 30) and normal control group (n = 30). T2DM group was fed with high fat and high cholesterol diet for 4 weeks and fasting for 16 hours. STZ, was injected intraperitoneally at 30 mg/kg for 16 hours. 72 hours later, blood samples were collected from tail vein to measure blood glucose. Blood glucose concentration (16.7mmol/L) was used as the standard for successful establishment of diabetic model. Serum 25 (OH) D levels were measured by Elisa. The expression of VDR mRNA and protein in liver tissue of two groups were detected by RT-PCR and Western blot respectively. Spearman correlation analysis was used to analyze the relationship between serum 25 (OH) D and FPG,F1ns,FFA,TG,TC,HOMA-IR in T2DM group. Results compared with NC group, the expression of VDR mRNA and protein in liver of T2DM group was increased [(2.15 卤0.25) vs (8.26 卤2.23), (0.317 卤0.085) vs (0.542 卤0.135)]. Serum 25 (OH) D level decreased [(43.6 卤12.3) vs (21.3 卤6.7) ng/ml] and serum FFA,TG,TC,LDL-C,FIns,HOMA-IR increased (P0.05). Correlation analysis showed that serum 25 (OH) D was negatively correlated with FFA,TG,FIns,HOMA-IR and positively correlated with HDL-C (P0.05). Conclusion the expression of VDR mRNA and protein in liver tissue of T2DM rats increased, while serum 25 (OH) D decreased. The decrease of serum 25 (OH) D may promote the expression of VDR in liver and further lead to abnormal metabolism of IR and glucose and lipid in liver.
【作者单位】：遵义医学院附属医院内分泌科;
【基金】：国家自然科学基金(81460168) 贵州省科学技术基金项目[黔科合J字LKZ(2013)19] 遵义医学院博士启动基金[(2012)F-574]
【分类号】：R587.1

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