联合使用甲状旁腺激素和钙剂对骨质疏松的防治有叠加作用

发布时间：2019-04-29 19:26

【摘要】：目的探索联合使用甲状旁腺激素和钙剂对去势大鼠诱导骨质疏松的防治作用。方法 50只健康雌性SD大鼠随机行假手术(Sham,N=10)和切除双侧卵巢(OVX,N=40)手术后,OVX大鼠随机的分成4组:OVX组、CA组、PTH组、PTH+CA组。术后第一天PTH组及PTH+CA开始给予药物治疗:PTH皮下注射(剂量60μg/kg,每周3次)。术后CA组及PTH+CA组大鼠饮食中加入钙剂,其余组大鼠普通饮食,直至手术后12周为止,12时所有大鼠处死取胫骨行Micro-CT、硬组织切片检测。结果 CA组、PTH组、PTH+CA组和OVX组相比,胫骨近端都有较高的BMD、BV/TV、Tb.Th、Tb.N、Conn.D、骨矿化沉积率(MAR)和较低的Tb.Sp,其中PTH+CA组大鼠胫骨有最高的BMD、BV/TV、Tb.Th、Tb.N、Conn.D、MAR和最低Tb.Sp。钙剂和PTH单独使用的效果明显低于他们联合使用对去卵巢大鼠骨质疏松的防治作用。结论联合使用甲状旁腺激素和钙剂对去势大鼠骨质疏松的防治有叠加作用
[Abstract]:Objective to explore the preventive and therapeutic effects of combined use of parathyroid hormone and calcium on osteoporosis induced by ovariectomized rats. Methods after sham operation (Sham,N=10) and bilateral ovariectomy (OVX,N=40) in 50 healthy female SD rats, OVX rats were randomly divided into 4 groups: OVX group, CA group and PTH group, PTH CA group. On the first day after operation, PTH group and PTH CA group were treated with PTH subcutaneous injection (60 渭 g / kg, 3 times a week). After operation, calcium was added to the diet of rats in CA group and PTH CA group, and normal diet was used in other groups until 12 weeks after operation. The tibia was taken from all rats at 12:00 and examined by Micro-CT, hard tissue section. Results in CA group, PTH CA group, compared with OVX group, the proximal tibia had higher BMD,BV/TV,Tb.Th,Tb.N,Conn.D, bone mineralization rate (MAR) and lower Tb.Sp, and PTH CA group had the highest BMD, in tibia. BV/TV,Tb.Th,Tb.N,Conn.D,MAR and minimum Tb.Sp. The effects of calcium and PTH alone on osteoporosis in ovariectomized rats were significantly lower than those in ovariectomized rats. Conclusion combined use of parathyroid hormone and calcium has a superimposed effect on the prevention and treatment of osteoporosis in ovariectomized rats.
【作者单位】：海口市第四人民医院骨科;海南医学院附属医院骨科;
【分类号】：R580

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