系统性红斑狼疮患者维生素D与T淋巴细胞亚群及α干扰素的相关性

发布时间：2019-05-19 08:53

【摘要】：目的探讨系统性红斑狼疮(SLE)患者维生素D水平与T淋巴细胞亚群及α干扰素(IFN-α)水平的相关性。方法选取SLE 80例(SLE组)分为缓解期40例(缓解期组)、活动期40例(活动期组),健康体检者30例作为健康对照组。酶联免疫吸附试验(ELISA)检测外周血浆中25(OH)D、IFN-α水平;流式细胞术(FCM)测定外周血中T淋巴细胞亚群水平。结果 (1)SLE组外周血25(OH)D水平低于健康对照组,差异有统计学意义(P0.05);活动期组外周血25(OH)D水平低于缓解期组,差异有统计学意义(P0.05);(2)SLE组外周血T淋巴细胞、Th淋巴细胞及Th/Ts与健康对照组相比显著降低,差异有统计学意义(P0.05),其中活动期组患者低于缓解期组,差异有统计学意义(P0.05);SLE组与健康对照组两组间Ts细胞计数相比差异无统计学意义(P0.05);(3)SLE组外周血IFN-α水平高于健康对照组,差异有统计学意义(P0.05),活动期组高于缓解期组,差异有统计学意义(P0.05);(4)SLE组外周血25(OH)D、T细胞、Th细胞计数及Th/Ts及IFN-α水平均与病情活动度具有相关性(P0.05);(5)SLE组外周血25(OH)D与T细胞、Th细胞计数、Th/Ts计数比值呈正相关,与IFN-α水平呈负相关(P0.05)。结论在SLE患者体内,维生素D水平与T淋巴细胞亚群及IFN-α水平具有显著的相关性,推测维生素D可能通过调控T淋巴细胞亚群动态平衡以及IFN-α水平引发机体的免疫异常。
[Abstract]:Objective to investigate the relationship between vitamin D level and T lymphocyte subsets and interferon 伪 (IFN- 伪) levels in patients with systemic lupus erythematosus (SLE). Methods 80 cases of SLE (SLE group) were divided into remission stage (n = 40), active stage (n = 40) and healthy physical examination (n = 30) as healthy control group. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of 25 (OH) D and IFN-伪 in peripheral plasma, and flow cytometry (FCM) was used to detect the level of T lymphocytes subsets in peripheral blood. Results (1) the level of 25 (OH) D in peripheral blood of SLE group was significantly lower than that of healthy control group (P 0.05), and the level of 25 (OH) D of peripheral blood of active group was lower than that of remission group (P 0.05). (2) compared with the healthy control group, the peripheral blood T lymphocytes, Th lymphocytes and Th/Ts in the SLE group were significantly lower than those in the healthy control group (P 0.05), and the patients in the active group were lower than those in the remission group. The difference was statistically significant (P 0.05). There was no significant difference in Ts cell count between SLE group and healthy control group (P 0.05). (3) the level of IFN- 伪 in peripheral blood of SLE group was significantly higher than that of healthy control group (P 0.05), and that of active group was higher than that of remission group (P 0.05). (4) in SLE group, the count of T cells, Th cells and the levels of Th/Ts and IFN- 伪 in peripheral blood 25 (OH) D were correlated with the activity of the disease (P 0.05). (5) in SLE group, 25 (OH) D of peripheral blood was positively correlated with T cell, Th cell count and Th/Ts count ratio, but negatively correlated with IFN- 伪 level (P 0.05). Conclusion Vitamin D level is significantly correlated with T lymphocyte subsets and IFN- 伪 levels in patients with SLE. It is speculated that vitamin D may induce immune abnormalities by regulating the dynamic balance of T lymphocytes subsets and the level of IFN- 伪.
【作者单位】：新疆医科大学第二附属医院风湿免疫科;
【基金】：新疆维吾尔自治区自然科学基金资助项目(编号:2015211C109)
【分类号】：R593.241

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