甲状腺激素联合多奈哌齐治疗对成年甲减大鼠额叶SNAP-25的影响

发布时间：2019-05-21 10:19

【摘要】：目的研究甲状腺激素联合多奈哌齐治疗对成年甲减大鼠额叶突触相关蛋白25(SNAP-25)的表达的影响。方法将50只成年健康SD大鼠随机分为5组:对照组(CON)、甲减组(Hypo)、多奈哌齐组(DON)、左甲状腺激素钠组(L-T4)、左甲状腺激素钠联合多奈哌齐组(L-T4+DON);除对照组外,其余4组通过含0.05%丙基硫氧嘧啶(PTU)饮用水喂养6周诱导成年期甲减大鼠模型;DON组、L-T4组、L-T4+DON组分别予多奈哌齐、左甲状腺激素钠、左甲状腺激素钠联合多奈哌齐治疗2周后,放射免疫法测定血清甲状腺激素水平,免疫组化法、Western blot法观察大鼠额叶SNAP-25的表达。结果与CON组相比,Hypo组大鼠血清三碘甲状腺原氨酸(T3)、四碘甲状腺原氨酸(T4)水平显著降低(P0.01),促甲状腺激素(TSH)显著上升(P0.01);额叶SNAP-25的表达水平显著高于CON组(P0.05)。L-T4组与CON组比较,血清T3、T4、TSH差异无统计学意义;SNAP-25的表达升高(P0.05)。DON组与CON组比较,血清T3、T4、TSH差异有统计学意义(P0.01);SNAP-25的表达升高(P0.05)。L-T4+DON组与CON组比较,血清T3、T4、TSH差异无统计学意义;SNAP-25的表达差异无统计学意义。结论甲状腺激素联合多奈哌齐治疗能有效地恢复成年甲减大鼠额叶SNAP-25的表达。
[Abstract]:Objective to study the effect of thyroid hormone combined with donepezil on the expression of synaptic protein 25 (SNAP-25) in frontolobe of adult hypothyroidism rats. Methods Fifty healthy adult SD rats were randomly divided into 5 groups: control group (CON), hypothyroidism group (Hypo), donepezil group (DON), left thyroid hormone sodium group (L-T4), left thyroid hormone sodium combined with donepezil group (L-T4 DON);). Except the control group, the other four groups were fed with 0.05% propylthiouracil (PTU) drinking water for 6 weeks to induce adult hypothyroidism rats. The serum thyroid hormone levels were measured by radioimmunoassay in DON group, L-T4 group and L-T4 DON group after 2 weeks of treatment with donepezil, left thyroid hormone sodium combined with donepezil, respectively. the serum thyroid hormone levels were measured by radioimmunoassays, and the serum thyroid hormone levels were measured by immuno-histochemical method. The expression of SNAP-25 in frontal lobe of rats was observed by Western blot assay. Results compared with CON group, the levels of serum triiodothyronine (T3) and tetraiodothyronine (T4) in Hypo group were significantly lower than those in Hypo group (P01), and the levels of thyrotropin (TSH) were significantly increased (P01). The expression level of SNAP-25 in frontal lobe was significantly higher than that in CON group (P 0.05). There was no significant difference in serum T3 and T4 between L-T4 group and CON group. The expression of SNAP-25 in). DON group was significantly higher than that in CON group (P01). Compared with CON group, there was no significant difference in serum T3 and T4, but there was no significant difference in the expression of SNAP-25 between L-T4 DON group and CON group. Conclusion thyroid hormone combined with donepezil can effectively restore the expression of SNAP-25 in the forehead of adult hypothyroidism rats.
【作者单位】：安徽医科大学第一附属医院老年病研究所;安徽医科大学第一附属医院老年内分泌科;安徽医科大学卫生毒理学教研室;
【基金】：国家自然科学基金面上项目(编号:81272152)
【分类号】：R581.2

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