血管内皮生长因子与色素上皮衍生因子在糖尿病猕猴视网膜病变早变早期的表达

发布时间：2019-05-22 01:43

【摘要】：目的检测糖尿病视网膜病变早期猕猴视网膜中血管内皮生长因子(VEGF)及色素上皮衍生因子(PEDF)的表达,探讨其意义。方法通过空腹血糖值糖化血红蛋白水平、眼底彩照情况及糖尿病病程,确定入选3只利用高脂饲料诱导成功且处于糖尿病视网膜病变早期的猕猴及3只年龄相当的健康猕猴。行实时荧光定量PCR及免疫组织化学检测,观察猕猴视网膜内VEGF和PEDF表达情况。结果处于糖尿病视网膜病变早期的猕猴视网膜内VEGF在mRNA表达水平(P0.05)及蛋白表达水平(P0.05)均明显高于对照组猕猴,糖尿病组猕猴视网膜内PEDF mRNA表达量相比对照组显著减少(P0.01),PEDF蛋白表达与其mRNA表达趋势一致(P0.05)。结论猕猴糖尿病视网膜早期,出现VEGF的上调和PEDF的下调,对糖尿病视网膜病变的发生具有一定的提示意义,可为早期糖尿病视网膜病变提供辅助诊断。
[Abstract]:Objective to detect the expression of vascular endothelial growth factor (VEGF) and pigment epithelial derived factor (PEDF) in the early stage of diabetic Retinopathy in rhesus monkeys. Methods according to fasting blood glucose glycosylated hemoglobin level, fundus color radiography and the course of diabetes mellitus, 3 rhesus monkeys and 3 healthy rhesus monkeys of the same age induced by high fat diet were selected. The expression of VEGF and PEDF in the retinal of rhesus monkeys was observed by real-time fluorescence quantitative PCR and immunohistochemistry. Results the expression of VEGF in the retinal of rhesus monkeys in the early stage of diabetic Retinopathy (P 0.05) was significantly higher than that of the control group (P 0.05). Compared with the control group, the expression of PEDF mRNA in the retinal of rhesus monkeys in diabetic group was significantly lower than that in the control group (P 0.01). The expression of), PEDF protein was consistent with the trend of mRNA expression in diabetic group (P 0.05). Conclusion the up-regulation of VEGF and the down-regulation of PEDF in the early stage of diabetic Retinopathy in rhesus monkeys have certain significance for the occurrence of diabetic Retinopathy and can provide auxiliary diagnosis for early Diabetic Retinopathy.
【作者单位】：四川农业大学动物医学院实验动物疾病模型研究室;国家实验猕猴种源基地;
【基金】：国家自然科学基金(31501200) 国家重大科学仪器设备开发专项(2013YQ49085906) 国家科技支撑计划课题(2014BAI03B01)~~
【分类号】：R587.2;R774.1

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