自身免疫性疾病患者血清抗白念珠菌烯醇化酶IgG型抗体的检测和分析
发布时间：2019-05-29 04:00
【摘要】：目的检测自身免疫性疾病患者血清中抗白念珠菌烯醇化酶(Eno)IgG型抗体表达情况。方法以重组白念珠菌Eno为包被抗原,建立ELISA检测方法并对反应条件优化,检测健康献血员对照组和自身免疫性疾病患者血清中抗白念珠菌Eno IgG型抗体。用Western blot法验证部分Eno抗体阳性血清的准确性。结果共检测自身免疫性疾病患者血清70份,其中抗白念珠菌Eno IgG型抗体阳性血清19份,总体阳性率为27.14%(19/70)。与对照组相比,自身免疫性疾病患者白念珠菌Eno IgG型抗体阳性率明显升高。其中,类风湿性关节炎患者白念珠菌Eno IgG型抗体阳性率为11.8%(2/17)、红斑狼疮患者阳性率为45.8%(11/24)。部分血清反应特异性得到Western blot验证。结论自身免疫性疾病患者血清抗白念珠菌Eno IgG型抗体阳性率较高,可能对白念珠菌Eno IgG型抗体应用于侵袭性念珠菌病诊断有干扰。
[Abstract]:Objective to detect the expression of anti-candida enolase (Eno) IGG antibody in serum of patients with autoimmune diseases. Methods using recombinant candida albicans Eno as coating antigen, a ELISA detection method was established and the reaction conditions were optimized. the serum anti-Eno antibody against candida albicans in healthy blood donors and patients with autoimmune diseases was detected. Western blot method was used to verify the accuracy of some Eno antibody positive serum. Results A total of 70 serum samples from patients with autoimmune diseases were detected, of which 19 were positive for anti-candida albicans Eno IGG antibody, the overall positive rate was 27.14% (19 鈮，
本文编号：2487626
[Abstract]:Objective to detect the expression of anti-candida enolase (Eno) IGG antibody in serum of patients with autoimmune diseases. Methods using recombinant candida albicans Eno as coating antigen, a ELISA detection method was established and the reaction conditions were optimized. the serum anti-Eno antibody against candida albicans in healthy blood donors and patients with autoimmune diseases was detected. Western blot method was used to verify the accuracy of some Eno antibody positive serum. Results A total of 70 serum samples from patients with autoimmune diseases were detected, of which 19 were positive for anti-candida albicans Eno IGG antibody, the overall positive rate was 27.14% (19 鈮，
本文编号：2487626
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