肿瘤坏死因子-α基因多态性与甲状腺相关眼病的相关性研究

发布时间：2019-06-10 03:26

【摘要】：目的:检测中国北方汉族人群是否存在TNF-α-863C/A、TNF-α-1031T/C、TNF-α-238G/A基因多态性,研究其与甲状腺相关眼病(thyroid associated ophthalmopathy,TAO)及疾病严重程度的相关性。方法:纳入研究对象464人,健康对照组152人,Graves'病无眼征组158人,TAO组154人。TAO组分为轻度52人,中重度55人,危及视力47人。采集外周抗凝血,磁珠法提取基因组DNA,TaqMan-MGB探针实时荧光定量PCR法检测每位研究对象3个位点的基因型。用卡方检验对数据进行统计学分析,Bonferroni法校正P值。结果:464 例研究对象中存在 TNF-α-863C/A、TNF-α-1031T/C、TNF-α-238G/A基因多态性。TNF-α-863C/A:中重度+危及视力TAO组AC+AA基因型频率高于健康组(Pc0.05),高于轻度TAO组(P0.05);中重度+危及视力TAO组等位基因A频率高于健康组(Pc0.05);男性GD无眼征+轻度TAO组等位基因A频率高于男性健康组(Pc0.05)。TNF-α-1031T/C:中重度+危及视力TAO组CT+CC基因型频率高于健康对照组、高于GD无眼征+轻度TAO组((Pc0.05))。危及视力TAO组CT+CC基因型频率高于轻度TAO组(Pc0.05),中重度+危及视力TAO组CT+CC基因型频率高于轻度TAO组(P0.05)。男性中重度+危及视力TAO组CT+CC基因型频率高于男性健康组(Pc0.05)。TNF-α-238G/A基因型、等位基因频率在组间分布差异无统计学意义。结论:中国北方汉族人群存在 TNF-α-863C/A、TNF-α-1031T/C、TNF-α-238G/A基因多态性。TNF-α-863C/A、TNF-α-1031T/C基因多态性与TAO的发病及疾病严重程度有相关性。TNF-α-863C/A、TNF-α-1031T/C可能是中国北方汉族人群TAO患者易感基因位点。TNF-α-238G/A基因多态性与中国北方汉族人群TAO的发病无关联。
[Abstract]:Aim: to detect the polymorphism of TNF- 伪-863C, TNF伪-1031T and TNF伪-238G/A genes in northern Chinese Han population, and to study the relationship between TNF伪-TNFA gene polymorphism and thyroid-associated ophthalmopathy (thyroid associated ophthalmopathy,TAO) and the severity of thyroid-associated ophthalmopathy (TNF伪-1031T). Methods: 464 subjects were included in the study, 152in healthy control group, 158in Graves' 's disease without eyesight group and 154in TAO group. TAO group was divided into mild group (n = 52), moderate and severe group (n = 55), endangering visual acuity (n = 47). Peripheral anticoagulation was collected and genomic DNA,TaqMan-MGB probe was extracted by magnetic beads to detect the genotype of 3 loci in each subject by real-time fluorescence quantitative PCR. Chi-square test was used to analyze the data and Bonferroni method was used to correct the P value. Results: TNF- 伪-863C 鈮，

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