甘露糖结合凝集素相关丝氨酸蛋白酶2与糖尿病肾病
发布时间：2019-06-22 08:19
【摘要】：目的:(1)应用血清蛋白质组学探讨糖尿病肾病(Diabetic Nephropathy,DN)发生发展机制。(2)验证甘露糖结合凝集素相关丝氨酸蛋白酶2(MBL associated serine protease 2,MASP2)在糖尿病肾病不同阶段的表达水平。(3)探讨甘露聚糖结合凝集素(mannose binding lectin,MBL)与2型糖尿病肾病的关系。方法:第一部分:入选年龄、性别匹配研究对象共4组,每组8人。组A为正常对照组,组B、C、D分别为2型糖尿病尿微量白蛋白排泄率(UAER)㩳30mg/g肌酐组、30mg/g肌酐≤UAER㩳300mg/g肌酐组、UAER≥300mg/g肌酐组。利用双向电泳对4组血清进行蛋白质分离。应用Image Master 2D Platinum分析软件(GE公司)分析四组之间差异表达的蛋白质点。挖取表达差异蛋白质点,进行基质辅助激光解附离子化飞行时间质谱(MALDI-TOF MS)分析,并与Swiss Prot数据库进行匹配鉴定蛋白质。第二部分:入选正常对照组(A组)54人,2型糖尿病患者尿微量白蛋白30mg/g肌酐组(B组)52人、尿微量白蛋白≥30mg/g肌酐且300mg/g肌酐组(C组)30人、尿微量白蛋白≥300mg/g肌酐组(D组)16人。采用双抗体夹心酶联免疫吸附实验(ELISA)来检测4组血清MASP2水平,验证血清蛋白质组实验结果。同时应用ELISA方法检测以上四组血清MBL水平。并分析MASP2和MBL与2型糖尿病慢性并发症的关系。结果:第一部分:血清蛋白质组学结果显示,在2型糖尿病B组患者中MASP2表达上调。第二部分:ELISA实验结果显示:(1)2型糖尿病组MASP2水平高于正常对照组(P0.05);(2)2型糖尿病B组B组和D组的MASP2表达高于C组(P0.05);(3)MBL在正常对照组与糖尿病组没有差别(P0.05),不同糖尿病肾病组没有差别(P0.05)。结论:(1)2型糖尿病患者甘露糖结合凝集素相关丝氨酸蛋白酶2水平升高。(2)MASP2可能参与2型糖尿病肾病的发生发展.
[Abstract]:Objective: (1) to investigate the mechanism of diabetic nephropathy (Diabetic Nephropathy,DN) by serum proteome. (2) to verify the expression of mannose binding lectin associated serine protease 2 (MBL associated serine protease 2, MASP2) in different stages of diabetic nephropathy. (3) to explore the relationship between mannan binding lectin (mannose binding lectin,MBL and type 2 diabetic nephropathy. Methods: in the first part, there were 4 groups of age and sex matching, 8 people in each group. Group A was normal control group, group B, C, D were type 2 diabetes mellitus urinary albumin excretion rate (UAER) 30mg/g creatine group, 30mg/g creatine 鈮，
本文编号：2504400
[Abstract]:Objective: (1) to investigate the mechanism of diabetic nephropathy (Diabetic Nephropathy,DN) by serum proteome. (2) to verify the expression of mannose binding lectin associated serine protease 2 (MBL associated serine protease 2, MASP2) in different stages of diabetic nephropathy. (3) to explore the relationship between mannan binding lectin (mannose binding lectin,MBL and type 2 diabetic nephropathy. Methods: in the first part, there were 4 groups of age and sex matching, 8 people in each group. Group A was normal control group, group B, C, D were type 2 diabetes mellitus urinary albumin excretion rate (UAER) 30mg/g creatine group, 30mg/g creatine 鈮，
本文编号：2504400
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