波动性甘油三酯水平及与高糖联合培养对人脐静脉内皮细胞的损伤作用
发布时间：2019-07-09 07:02
【摘要】：目的:通过体外研究模拟餐后甘油三酯异常以及伴有的血糖异常对人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)的损伤作用,探讨餐后状态下甘油三酯、甘油三酯波动、高血糖并存对血管内皮的损伤作用。方法:1以人脐静脉内皮细胞(HUVEC)为研究对象,体外培养至第六代并应用不含血清的1640完全培养基饥饿24小时已达到同步化后用于试验。2从医院收集人的正常血清及高甘油三酯血清(HTGBS)并配比为所需浓度,并进行除菌、灭活处理。其他指标配比为相近浓度。血清中的甘油三酯(TG)、胆固醇(TC)、葡萄糖(GLU)、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)等指标均由医院检验科全自动生化分析仪检测。分装并储存在-80℃冰箱中。3实验共10组,给予以下条件,共作用3天。1组:葡萄糖浓度5.5 mmol/L+TG 1.56 mmol/L。2组:葡萄糖浓度5.5 mmol/L+TG 3.45 mmol/L,3组:葡萄糖浓度5.6 mmol/L+TG 5.52 mmol/L,4组:葡萄糖浓度5.5 mmol/L+TG 1.56 mmol/L(8小时)与3.45 mmol/L(16小时)交替,5组:葡萄糖浓度5.5 mmol/L+TG 1.56 mmol/L(8小时)与5.52 mmol/L(16小时)交替,6组:葡萄糖浓度25 mmol/L+TG 1.56 mmol/L,7组:葡萄糖浓度25 mmol/L+TG 3.45 mmol/L,8组:葡萄糖浓度25 mmol/L+TG 5.52 mmol/L,9组:葡萄糖浓度25 mmol/L+TG 1.56 mmol/L(8小时)与3.45 mmol/L(16小时)交替,10组:葡萄糖浓度25 mmol/L+TG 1.56 mmol/L(8小时)与5.52 mmol/L(16小时)交替。结束后换用葡萄糖浓度5.5 mmol/L+TG 1.56 mmol/L培养8小时,测定各组细胞活力(MTT)指标,收取上清液测单核细胞趋化蛋白(monocyte chemoattractant protein-1,MCP-1)、血管内皮细胞间粘附分子vascular cell adhesion molecule,VCAM-1)、细胞间粘附分子(interscular cell adhesionmolecule,ICAM)、C反应蛋白(C-reactive protein,CRP)、丙二醛malondiadehyde,MDA)、超氧化物歧化酶(superoxide sismutase,SOD)、一氧化氮(nitric oxide,NO)。4统计学分析:符合正态分布计量资料以x±s表示,采用SPSS13.0软件进行统计学分析,多组间数据比较用单因素方差分析,两两比较用LSD检验,P0.05为差异有统计学意义。结果:1 MTT:中浓度甘油三酯波动组大于高浓度波动组的OD值(P0.05)。相同葡萄糖浓度情况下,与低浓度甘油三酯组比较,持续中浓度甘油三酯组OD值升高,而持续高浓度甘油三酯组OD值降低。在相同的甘油三酯作用下,高糖组要比低糖组的OD值低,单纯高糖组的OD值大于单纯高浓度甘油三酯组而小于单纯中浓度高甘油三酯组,而高浓度葡萄糖与高浓度甘油三酯联合培养组含量最低。同等程度的甘油三酯波动,高糖组的OD值要低于低糖组。2上清液CRP水平:相同葡萄糖浓度下,持续高甘油三酯及波动性高甘油三脂组测得CRP均数均高于低甘油三酯组。在低葡萄糖浓度组,与低浓度甘油三酯组相比,其他各组CRP均升高,甘油三酯波动组较等水平的高甘油三脂组低,但无明显差别。在联合高浓度葡萄糖组中,与低浓度甘油三脂组相比,其他各组CRP升高,但波动性甘油三酯组较其无明显差异。在同等甘油三酯水平下,高糖组CRP较低糖组高。单纯高糖组CRP较单纯高浓度甘油三酯组的CRP低,而高浓度葡萄糖与高浓度甘油三酯联合培养组含量最高。同等程度的甘油三酯波动,高糖组的CRP含量要大于低糖组。3上清液ICAM含量:相同葡萄糖浓度下,持续高甘油三酯及波动性高甘油三脂组测得ICAM均数均高于低甘油三酯组。在低葡萄糖浓度组,波动性中浓度甘油三酯组与低浓度甘油三酯组相比无明显差异,其他各组ICAM与其相比均明显升高。在联合高浓度葡萄糖组中,与低浓度甘油三脂组相比,持续高甘油三脂组ICAM均数升高,但波动性甘油三酯组较其无明显差异。在同等甘油三酯水平下,高糖组ICAM较低糖组高。单纯高糖组ICAM较单纯高浓度甘油三酯组的ICAM低,而高浓度葡萄糖 与高浓度甘油三酯联合培养组含量最高。同等程度的甘油三酯波动,高糖组的ICAM含量要大于低糖组。4上清液VCAM含量:相同葡萄糖浓度下,持续高甘油三酯及波动性高甘油三脂组测得VCAM均数均高于低甘油三酯组。在低葡萄糖浓度组,波动性高浓度甘油三酯组和持续高浓度甘油三酯组与低浓度甘油三酯组相比明显升高,其他各组VCAM与其相比无明显差异。在联合高浓度葡萄糖组中,与低浓度甘油三脂组相比,波动性中浓度甘油三酯组较其无明显差异,而其他组较其明显升高。在同等甘油三酯水平下,高糖组CVAM较低糖组高。单纯高糖组VCAM较单纯高浓度甘油三酯组的VCAM低,而高浓度葡萄糖与高浓度甘油三酯联合培养组含量最高。同等程度的甘油三酯波动,高糖组的VCAM含量要大于低糖组。5上清液MCP-1含量:相同葡萄糖浓度下,持续高甘油三酯及波动性高甘油三脂组测得MCP-1均数均高于低甘油三酯组,波动性高浓度甘油三酯组和持续高浓度甘油三酯组与低浓度甘油三酯组相比明显升高,其他组MCP-1与其相比无明显差异。在高浓度葡萄糖组中,持续高浓度甘油三酯组与低浓度甘油三酯组比较明显升高,其他组较其无明显差异。在同等甘油三酯水平下,高糖组MCP-1较低糖组高。单纯高糖组MCP-1较单纯高浓度甘油三酯组低,而高浓度葡萄糖与高浓度甘油三酯联合培养组含量最高。同等程度的甘油三酯波动,高糖组的MCP-1含量要大于低糖组。6上清液MDA含量:相同葡萄糖浓度下,持续高甘油三酯及波动性高甘油三脂均可不同程度增加上清液中炎性产物MDA的表达。但波动性甘油三脂组较其无明显差异。在同等甘油三酯水平下,高糖组MDA较低糖组高。单纯高糖组MDA较单纯高浓度甘油三酯组低,而高浓度葡萄糖与高浓度甘油三酯联合培养组含量最高。同等程度的甘油三酯波动,高糖组的MDA含量要大于低糖组。7在相同葡萄糖浓度下,持续高甘油三酯及波动性高甘油三脂均可降低上清液中保护性因子SOD、NO含量,在低葡萄糖浓度组,各组与低浓度甘油三酯组相比明显降低。在联合高浓度葡萄糖组中,与低浓度甘油三脂组相比降低,但波动性高浓度甘油三酯组和持续高浓度甘油三酯组较其明显降低。在同等甘油三酯水平下,高糖组NO和SOD较低糖组低。单纯高糖组NO、SOD较单纯高浓度甘油三酯组高,而高浓度葡萄糖与高浓度甘油三酯联合培养组含量最低。同等程度的甘油三酯波动,高糖组的SOD、NO含量要大于低糖组。结论:1在相同葡萄糖浓度时,波动性甘油三酯和持续中浓度甘油三酯均促进细胞增殖,且低糖并中浓度甘油三酯波动的促增殖作用最大,持续高浓度甘油三酯均抑制细胞增殖,且高糖联合持续高浓度甘油三酯的抑制细胞增殖的作用最强。单纯高浓度甘油三脂对细胞增殖的抑制作用大于单纯高糖。2波动性高甘油三酯和持续高甘油三酯对人脐静脉内皮细胞均有炎性损伤,但波动性高甘油三酯对细胞的损伤不大于持续高甘油三酯。单纯高水平的高甘油三脂对细胞的损伤作用大于单纯高糖,高糖联合持续高浓度的甘油三酯对细胞的作用最大,同等甘油三酯波动时,高葡萄糖的炎性损伤作用大于低葡萄糖。3波动性高甘油三酯和持续高甘油三酯导致人脐静脉内皮细胞抗氧化能力减弱;但波动性高甘油三酯对细胞抗氧化能力的影响不大于持续高甘油三酯。单纯高水平的高甘油三脂对细胞的抗氧化能力的减弱作用大于单纯高糖,高糖联合持续高浓度的甘油三酯对细胞的抗氧化能力的减弱作用最大。同样的甘油三酯波动时时,高葡萄糖的炎性损伤作用大于低葡萄糖。4波动性高甘油三酯和持续高甘油三酯导致人脐静脉内皮细胞功能损伤(NO含量降低),但波动性高甘油三酯对细胞的影响不大于持续高甘油三酯。单纯高水平的高甘油三脂对细胞内皮功能的损伤作用大于单纯高糖,高糖联合持续高浓度的甘油三酯对HUVEC功能的损伤作用最大。同样的甘油三酯波动时时,高葡萄糖对HUVEC的损伤作用大于低葡萄糖。
[Abstract]:Objective: To study the effects of postprandial triglyceride and abnormal blood glucose on human umbilical vein endothelial cell (HUVEC) by in vitro study. Methods:1 Human umbilical vein endothelial cell (HUVEC) was used as the research object. The cells were cultured in vitro to the sixth generation and the serum-free 1640 full-medium starvation was applied for the test after 24 hours of synchronization. The normal serum and high triglyceride serum (HTGBS) of the human were collected from the hospital and matched to the desired concentration, and the sterilization and inactivation treatment were carried out. The proportion of other indexes is similar concentration. The indexes of triglyceride (TG), cholesterol (TC), glucose (GLU), low density lipoprotein (LDL) and high-density lipoprotein (HDL) in the serum are detected by the full-automatic biochemical analyzer of the hospital laboratory. The concentration of glucose was 5.5 mmol/ L + TG 1.56 mmol/ L, and the glucose concentration was 5.5mmol/ L + TG 3.45mmol/ L, and the glucose concentration was 5.6 mmol/ L + TG 5.52 mmol/ L. The glucose concentration was 5.5 mmol/ L + TG 1.56 mmol/ L (8 hours) and 3.45 mmol/ L (16 hours),5 groups: glucose concentration of 5.5 mmol/ L + TG 1.56 mmol/ L (8 hours) and 5.52 mmol/ L (16 hours),6 groups: glucose concentration of 25 mmol/ L + TG 1.56 mmol/ L,7 groups: glucose concentration of 25 mmol/ L + TG 3.45 mmol/ L,8 groups: glucose concentration of 25 mmol/ L + TG 5.52 mmol/ L,9 groups: The glucose concentration was 25 mmol/ L + TG 1.56 mmol/ L (8 hours) and 3.45 mmol/ L (16 hours), and the glucose concentration was 25 mmol/ L + TG 1.56 mmol/ L (8 hours) and 5.52 mmol/ L (16 hours). After the end, the cell viability (MTT) index was measured with the glucose concentration of 5.5 mmol/ L + TG 1.56 mmol/ L for 8 hours, and the cell adhesion molecule (ICAM-1), the vascular endothelial cell adhesion molecule (VCAM-1) and the inter-cell adhesion molecule (ICAM) were collected. Statistical analysis of C-reactive protein (CRP), malondiadehyde (MDA), superoxide dismutase (SOD) and nitric oxide (NO). One-factor analysis of variance was used to compare the data among the groups, and the two comparisons were compared with the LSD, and the difference between the two groups was statistically significant. Results:1 MTT: The concentration of the medium-concentration triglyceride was higher than that of the high-concentration fluctuation group (P0.05). In that case of the same glucose concentration, the OD value of the continuous medium-concentration triglyceride group was increase while the OD value of the high-concentration triglyceride group was decreased in comparison with the low-concentration triglyceride group. Under the same triglyceride, the OD value of the high-sugar group was lower than that of the low-sugar group, the OD value of the simple high-sugar group was higher than that of the pure high-concentration triglyceride group, and the content of the high-concentration glucose and the high-concentration triglyceride combined culture group was the lowest. In the same degree of triglyceride fluctuation, the OD value of the high-sugar group was lower than that of the low-sugar group. The CRP level of the supernatant of the supernatant was higher than that of the low-triglyceride group at the same glucose concentration. In the low glucose concentration group, compared with the low-concentration triglyceride group, the levels of CRP in the other groups increased, and the triglyceride fluctuation group was lower than that of the higher triglyceride group, but there was no significant difference. In the combined high-concentration glucose group, the CRP in other groups increased compared with the low-concentration triglyceride group, but there was no significant difference in the volatile triglyceride group. In that same triglyceride level, the CRP in the high-sugar group was higher than that of the low-sugar group. The CRP of pure high-sugar group was lower than that of pure high-concentration triglyceride group, while the high-concentration glucose combined with high-concentration triglyceride was the highest. In the same degree of triglyceride fluctuation, the content of CRP in the high-sugar group was higher than that of the low-sugar group. The content of ICAM-1 in the supernatant of the supernatant was higher than that of the low-triglyceride group at the same glucose concentration. In the low glucose concentration group, there was no significant difference between the concentration triglyceride group and the low-concentration triglyceride group in the fluctuation, and the ICAM-1 in the other groups was significantly higher than that of the low-concentration triglyceride group. In the combined high-concentration glucose group, there was no significant difference in the number of ICAM-1 in the continuous high-fat group compared with the low-concentration triglyceride group. At the same triglyceride level, the ICAM-1 of the high-sugar group was higher than that of the low-sugar group. The ICAM-1 of the simple high-sugar group was lower than that of the pure high-concentration triglyceride group, while the high-concentration glucose and the high-concentration triglyceride combined culture group were the highest. The content of ICAM-1 in the high-sugar group was higher than that of the low-sugar group, and the content of VCAM in the high-sugar group was higher than that of the low-triglyceride group at the same glucose concentration. In the low glucose concentration group, the volatile high-concentration triglyceride group and the continuous high-concentration triglyceride group were significantly increased compared with the low-concentration triglyceride group, and the other groups of VCAM had no significant difference compared with the low-concentration triglyceride group. In the combined high-concentration glucose group, there was no significant difference between the low-concentration triglyceride group and the low-concentration triglyceride group, and the other group was significantly higher than that in the other group. In that same triglyceride level, the CVIAM of the high-sugar group was higher than that of the low-sugar group. VCAM of pure high-sugar group was lower than that of pure high-concentration triglyceride group, while the content of high-concentration glucose and high-concentration triglyceride was the highest. The content of MCP-1 in the high-sugar group was higher than that of the low-triglyceride group. The content of MCP-1 in the high-sugar group was higher than that of the low-triglyceride group at the same glucose concentration. The volatile high-concentration triglyceride group and the continuous high-concentration triglyceride group increased significantly compared with the low-concentration triglyceride group, and the other groups of the MCP-1 had no significant difference compared with the low-concentration triglyceride group. In the high-concentration glucose group, the high-concentration triglyceride group and the low-concentration triglyceride group were significantly increased, and there was no significant difference in other groups. In the same triglyceride level, the high sugar group MCP-1 was higher in the lower sugar group. In the simple high-sugar group, MCP-1 was lower than that of the pure high-concentration triglyceride group, while the high-concentration glucose and the high-concentration triglyceride combined culture group were the highest. The content of MCP-1 in the high-sugar group was higher than that of the low-sugar group. The content of MDA in the supernatant of the supernatant was higher than that of the low-sugar group. However, there was no significant difference between the volatility and the fat group. In that same triglyceride level, the MDA in the high-sugar group was higher than that of the low-sugar group. The content of MDA in the high-sugar group was lower than that of the pure high-concentration triglyceride group, while the high-concentration glucose and the high-concentration triglyceride combined culture group were the highest. the content of MDA in the high-sugar group is higher than that of the low-sugar group under the same glucose concentration, and the content of the protective factor SOD and the NO in the supernatant can be reduced, and the content of the SOD and the NO in the low-glucose concentration group can be reduced, The groups are obviously reduced compared with the low-concentration triglyceride group. In the combined high-concentration glucose group, the low-concentration triglyceride group and the continuous high-concentration triglyceride group were significantly reduced compared to the low-concentration triglyceride group. In that same triglyceride level, the NO and SOD of the high-sugar group were lower than that of the low-sugar group. The content of NO and SOD in the pure high-sugar group was higher than that of the pure high-concentration triglyceride group, while the content of high-concentration glucose and high-concentration triglyceride combined culture group was the lowest. And the content of SOD and NO in the high-sugar group is higher than that of the low-sugar group. Conclusion:1 At the same glucose concentration, both the volatile triglyceride and the continuous middle-concentration triglyceride promote the proliferation of the cell, and the proliferation of the low-sugar and medium-concentration triglyceride is the largest, and the high-concentration triglyceride can inhibit the proliferation of the cells. And the effect of high glucose and high concentration of triglyceride in inhibiting cell proliferation is the strongest. The inhibitory effect of pure high-concentration triglyceride on the proliferation of human umbilical vein was higher than that of pure high-sugar. the only high-level high-fat-fat-to-cell damage action is greater than that of pure high-sugar and high-sugar and high-concentration triglyceride to the cell, and when the equivalent triglyceride fluctuates, The inflammatory injury of high glucose is greater than that of low glucose. The high triglyceride and persistent high triglyceride of the high glucose result in the decrease of the anti-oxidation ability of human umbilical vein endothelial cells, but the effect of the high triglyceride on the anti-oxidation ability of the cells is not more than the continuous high triglyceride. The effect of high-level high-fat-fat on the anti-oxidation ability of the cells is greater than that of pure high-sugar, high-sugar and high-concentration triglyceride to the anti-oxidation ability of the cells. in that same triglyceride fluctuation, the inflammatory injury of high glucose is great than that of low-glucose.4-volatile high-triglyceride and persistent high-triglyceride result in the functional damage of human umbilical vein endothelial cells (NO content decrease), However, that effect of volatile high triglyceride on the cell is not great than the persistent high triglyceride. The effect of high-level high-fat-fat on the function of the endothelial function of the HUVEC was higher than that of pure high-sugar, high-sugar and high-concentration triglyceride, and the damage to HUVEC was the most. In the same triglyceride fluctuation, the damage of high glucose to HUVEC was greater than that of low glucose.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R589
本文编号：2511953
[Abstract]:Objective: To study the effects of postprandial triglyceride and abnormal blood glucose on human umbilical vein endothelial cell (HUVEC) by in vitro study. Methods:1 Human umbilical vein endothelial cell (HUVEC) was used as the research object. The cells were cultured in vitro to the sixth generation and the serum-free 1640 full-medium starvation was applied for the test after 24 hours of synchronization. The normal serum and high triglyceride serum (HTGBS) of the human were collected from the hospital and matched to the desired concentration, and the sterilization and inactivation treatment were carried out. The proportion of other indexes is similar concentration. The indexes of triglyceride (TG), cholesterol (TC), glucose (GLU), low density lipoprotein (LDL) and high-density lipoprotein (HDL) in the serum are detected by the full-automatic biochemical analyzer of the hospital laboratory. The concentration of glucose was 5.5 mmol/ L + TG 1.56 mmol/ L, and the glucose concentration was 5.5mmol/ L + TG 3.45mmol/ L, and the glucose concentration was 5.6 mmol/ L + TG 5.52 mmol/ L. The glucose concentration was 5.5 mmol/ L + TG 1.56 mmol/ L (8 hours) and 3.45 mmol/ L (16 hours),5 groups: glucose concentration of 5.5 mmol/ L + TG 1.56 mmol/ L (8 hours) and 5.52 mmol/ L (16 hours),6 groups: glucose concentration of 25 mmol/ L + TG 1.56 mmol/ L,7 groups: glucose concentration of 25 mmol/ L + TG 3.45 mmol/ L,8 groups: glucose concentration of 25 mmol/ L + TG 5.52 mmol/ L,9 groups: The glucose concentration was 25 mmol/ L + TG 1.56 mmol/ L (8 hours) and 3.45 mmol/ L (16 hours), and the glucose concentration was 25 mmol/ L + TG 1.56 mmol/ L (8 hours) and 5.52 mmol/ L (16 hours). After the end, the cell viability (MTT) index was measured with the glucose concentration of 5.5 mmol/ L + TG 1.56 mmol/ L for 8 hours, and the cell adhesion molecule (ICAM-1), the vascular endothelial cell adhesion molecule (VCAM-1) and the inter-cell adhesion molecule (ICAM) were collected. Statistical analysis of C-reactive protein (CRP), malondiadehyde (MDA), superoxide dismutase (SOD) and nitric oxide (NO). One-factor analysis of variance was used to compare the data among the groups, and the two comparisons were compared with the LSD, and the difference between the two groups was statistically significant. Results:1 MTT: The concentration of the medium-concentration triglyceride was higher than that of the high-concentration fluctuation group (P0.05). In that case of the same glucose concentration, the OD value of the continuous medium-concentration triglyceride group was increase while the OD value of the high-concentration triglyceride group was decreased in comparison with the low-concentration triglyceride group. Under the same triglyceride, the OD value of the high-sugar group was lower than that of the low-sugar group, the OD value of the simple high-sugar group was higher than that of the pure high-concentration triglyceride group, and the content of the high-concentration glucose and the high-concentration triglyceride combined culture group was the lowest. In the same degree of triglyceride fluctuation, the OD value of the high-sugar group was lower than that of the low-sugar group. The CRP level of the supernatant of the supernatant was higher than that of the low-triglyceride group at the same glucose concentration. In the low glucose concentration group, compared with the low-concentration triglyceride group, the levels of CRP in the other groups increased, and the triglyceride fluctuation group was lower than that of the higher triglyceride group, but there was no significant difference. In the combined high-concentration glucose group, the CRP in other groups increased compared with the low-concentration triglyceride group, but there was no significant difference in the volatile triglyceride group. In that same triglyceride level, the CRP in the high-sugar group was higher than that of the low-sugar group. The CRP of pure high-sugar group was lower than that of pure high-concentration triglyceride group, while the high-concentration glucose combined with high-concentration triglyceride was the highest. In the same degree of triglyceride fluctuation, the content of CRP in the high-sugar group was higher than that of the low-sugar group. The content of ICAM-1 in the supernatant of the supernatant was higher than that of the low-triglyceride group at the same glucose concentration. In the low glucose concentration group, there was no significant difference between the concentration triglyceride group and the low-concentration triglyceride group in the fluctuation, and the ICAM-1 in the other groups was significantly higher than that of the low-concentration triglyceride group. In the combined high-concentration glucose group, there was no significant difference in the number of ICAM-1 in the continuous high-fat group compared with the low-concentration triglyceride group. At the same triglyceride level, the ICAM-1 of the high-sugar group was higher than that of the low-sugar group. The ICAM-1 of the simple high-sugar group was lower than that of the pure high-concentration triglyceride group, while the high-concentration glucose and the high-concentration triglyceride combined culture group were the highest. The content of ICAM-1 in the high-sugar group was higher than that of the low-sugar group, and the content of VCAM in the high-sugar group was higher than that of the low-triglyceride group at the same glucose concentration. In the low glucose concentration group, the volatile high-concentration triglyceride group and the continuous high-concentration triglyceride group were significantly increased compared with the low-concentration triglyceride group, and the other groups of VCAM had no significant difference compared with the low-concentration triglyceride group. In the combined high-concentration glucose group, there was no significant difference between the low-concentration triglyceride group and the low-concentration triglyceride group, and the other group was significantly higher than that in the other group. In that same triglyceride level, the CVIAM of the high-sugar group was higher than that of the low-sugar group. VCAM of pure high-sugar group was lower than that of pure high-concentration triglyceride group, while the content of high-concentration glucose and high-concentration triglyceride was the highest. The content of MCP-1 in the high-sugar group was higher than that of the low-triglyceride group. The content of MCP-1 in the high-sugar group was higher than that of the low-triglyceride group at the same glucose concentration. The volatile high-concentration triglyceride group and the continuous high-concentration triglyceride group increased significantly compared with the low-concentration triglyceride group, and the other groups of the MCP-1 had no significant difference compared with the low-concentration triglyceride group. In the high-concentration glucose group, the high-concentration triglyceride group and the low-concentration triglyceride group were significantly increased, and there was no significant difference in other groups. In the same triglyceride level, the high sugar group MCP-1 was higher in the lower sugar group. In the simple high-sugar group, MCP-1 was lower than that of the pure high-concentration triglyceride group, while the high-concentration glucose and the high-concentration triglyceride combined culture group were the highest. The content of MCP-1 in the high-sugar group was higher than that of the low-sugar group. The content of MDA in the supernatant of the supernatant was higher than that of the low-sugar group. However, there was no significant difference between the volatility and the fat group. In that same triglyceride level, the MDA in the high-sugar group was higher than that of the low-sugar group. The content of MDA in the high-sugar group was lower than that of the pure high-concentration triglyceride group, while the high-concentration glucose and the high-concentration triglyceride combined culture group were the highest. the content of MDA in the high-sugar group is higher than that of the low-sugar group under the same glucose concentration, and the content of the protective factor SOD and the NO in the supernatant can be reduced, and the content of the SOD and the NO in the low-glucose concentration group can be reduced, The groups are obviously reduced compared with the low-concentration triglyceride group. In the combined high-concentration glucose group, the low-concentration triglyceride group and the continuous high-concentration triglyceride group were significantly reduced compared to the low-concentration triglyceride group. In that same triglyceride level, the NO and SOD of the high-sugar group were lower than that of the low-sugar group. The content of NO and SOD in the pure high-sugar group was higher than that of the pure high-concentration triglyceride group, while the content of high-concentration glucose and high-concentration triglyceride combined culture group was the lowest. And the content of SOD and NO in the high-sugar group is higher than that of the low-sugar group. Conclusion:1 At the same glucose concentration, both the volatile triglyceride and the continuous middle-concentration triglyceride promote the proliferation of the cell, and the proliferation of the low-sugar and medium-concentration triglyceride is the largest, and the high-concentration triglyceride can inhibit the proliferation of the cells. And the effect of high glucose and high concentration of triglyceride in inhibiting cell proliferation is the strongest. The inhibitory effect of pure high-concentration triglyceride on the proliferation of human umbilical vein was higher than that of pure high-sugar. the only high-level high-fat-fat-to-cell damage action is greater than that of pure high-sugar and high-sugar and high-concentration triglyceride to the cell, and when the equivalent triglyceride fluctuates, The inflammatory injury of high glucose is greater than that of low glucose. The high triglyceride and persistent high triglyceride of the high glucose result in the decrease of the anti-oxidation ability of human umbilical vein endothelial cells, but the effect of the high triglyceride on the anti-oxidation ability of the cells is not more than the continuous high triglyceride. The effect of high-level high-fat-fat on the anti-oxidation ability of the cells is greater than that of pure high-sugar, high-sugar and high-concentration triglyceride to the anti-oxidation ability of the cells. in that same triglyceride fluctuation, the inflammatory injury of high glucose is great than that of low-glucose.4-volatile high-triglyceride and persistent high-triglyceride result in the functional damage of human umbilical vein endothelial cells (NO content decrease), However, that effect of volatile high triglyceride on the cell is not great than the persistent high triglyceride. The effect of high-level high-fat-fat on the function of the endothelial function of the HUVEC was higher than that of pure high-sugar, high-sugar and high-concentration triglyceride, and the damage to HUVEC was the most. In the same triglyceride fluctuation, the damage of high glucose to HUVEC was greater than that of low glucose.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R589
【参考文献】
相关期刊论文 前2条
1 陈玲,柴大军,林新霖,林金秀,康晴,许昌声,陈树强;高分辨率超声研究餐后高甘油三酯血症对正常人血管内皮功能的损伤[J];中国超声医学杂志;2001年11期
2 张力辉;周红;杨振华;郝咏梅;王瑞英;;2型糖尿病患者餐后脂代谢状态与大血管并发症的关系[J];中国老年学杂志;2007年20期
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