茶多酚对慢性酒精中毒肝损伤影响的实验研究

发布时间：2019-07-09 19:09

【摘要】：目的探讨茶多酚在预防慢性酒精中毒对肝损伤方面的作用及其可能的机制。方法将40只雄性昆明小鼠随机分成正常对照组、酒精组、茶多酚低剂量组和茶多酚高剂量组。酒精组、茶多酚组连续90 d喂服30%酒精,8 h后再分别喂服纯水,浓度1%、2%的茶多酚悬液。正常对照组以同样方式和剂量每天2次喂服纯水。末次灌胃24 h检测血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)的含量和肝组织中超氧化物歧化酶SOD、丙二醛(MDA)、组织内活性氧类物质(ROS)、谷胱甘肽过氧化物酶(GSH-px)的水平;同时,取肝组织行免疫组织化学显示Bcl-2和Bax的表达。结果与正常对照组比较,酒精损伤组肝细胞Bax阳性表达均明显升高(P0.01),肝细胞Bcl-2阳性表达明显降低(P0.01)。茶多酚喂服的两组有所改善。结论慢性酒精中毒可以引起肝损伤,而喂服茶多酚能减轻损伤程度。
[Abstract]:Objective to investigate the role of tea polyphenols in preventing chronic alcoholism from liver injury and its possible mechanism. Methods 40 male Kunming mice were randomly divided into normal control group, alcoholic group, tea polyphenol low dose group and tea polyphenol high dose group. In the alcohol group, the tea polyphenols group was fed with 30% alcohol for 90 days, and then the tea polyphenols suspension was fed with pure water for 8 hours at the concentration of 1% and 2% respectively. The normal control group was fed with pure water twice a day in the same way and dose. The content of glutamic oxaloacetic transaminase (AST),) glutamic-pyruvic transaminase (ALT) in serum and the level of (ROS), glutamic-pyruvic transaminase (GSH-px) in liver tissue were measured by 24 h of the last intragastric administration. At the same time, the expression of Bcl-2 and Bax in liver tissue was detected by immunohistochemistry. Results compared with the normal control group, the positive expression of Bax in hepatocytes of alcohol injury group was significantly higher than that of normal control group (P 0.01), and the positive expression of Bcl-2 in hepatocytes was significantly decreased (P 0.01). The two groups fed with tea polyphenols were improved. Conclusion chronic alcoholism can cause liver injury, and tea polyphenols can reduce the degree of injury.
【作者单位】：中南大学基础医学院院组织学系与胚胎学系;湘南学院基础医学院/南岭药用资源研究所;湘南学院临床学院;南方医科大学基础医学院;
【基金】：湖南省教育厅课题(15C1276) 湘南学院大学生研究性学习与创新性实验项目(湘南学院校发【2013】155号);湘南学院“橘井泉香”科技文化节项目(2014KJJ06) 国家级大学生研究性学习和创新性实验项目(教高司函[2015]41号) 湖南省大学生研究性学习和创新性实验项目(湘教通[2015]269号)
【分类号】：R595.6

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