菊粉联合游泳训练对代谢综合征大鼠骨骼肌胰岛素受体底物1及葡萄糖转运子4表达的影响
发布时间：2019-08-02 11:47
【摘要】：目的观察菊粉联合游泳训练对代谢综合征(MS)大鼠骨骼肌胰岛素受体底物1(IRS-1)、葡萄糖转运体子4(GluT4)表达的影响。方法高糖高脂高盐喂养结合腹腔注射STZ建立MS大鼠模型,干预8周后检测各组FBG、HbA_1c及FIns,计算HOMA-IR;HE染色法观察各组肝脏病理变化;免疫印迹及免疫组织化学法检测大鼠骨骼肌IRS-1、GluT4表达。结果与正常饮食对照组比较,模型组FBG、HbA_1c、FIns及HOMA-IR升高(P0.01),肝脏出现非酒精性脂肪性肝病(NAFLD)病理改变,骨骼肌IRS-1、GluT4表达降低(P0.01)。与模型组比较,菊粉联合游泳训练组FBG、HbA_1c、FIns及HOMA-IR降低(P0.01),NAFLD减轻,骨骼肌IRS-1、GluT4表达升高(P0.01)。结论菊粉联合游泳训练降低了MS大鼠FBG、HbA_1c及FIns水平,减轻了NAFLD病理改变,改善IR,其机制可能与上调骨骼肌IRS-1、GluT4表达有关。
[Abstract]:Objective to observe the effect of inulin combined with swimming training on the expression of insulin receptor substrate 1 (IRS-1) and glucose transporter 4 (GluT4) in skeletal muscle of (MS) rats with metabolic syndrome. Methods MS rat model was established by high glucose, high fat and high salt feeding combined with intraabdominal injection of STZ. After 8 weeks of intervention, FBG,HbA_1c and FIns, were detected by HOMA-IR;HE staining, and the expression of IRS-1,GluT4 in skeletal muscle of rats was detected by immunoblotting and immunohistochemistry. Results compared with the normal diet control group, FBG,HbA_1c,FIns and HOMA-IR in the model group were increased (P01), (NAFLD) pathological changes were found in the liver, and the expression of IRS-1,GluT4 in skeletal muscle was decreased (P01). Compared with the model group, FBG,HbA_1c,FIns and HOMA-IR in the inulin combined swimming training group decreased (P 0.01), NAFLD) and the expression of IRS-1,GluT4 in skeletal muscle increased (P 0.01). Conclusion inulin combined swimming training can decrease the levels of FBG,HbA_1c and FIns and alleviate the pathological changes of NAFLD in MS rats. The mechanism of improving IR, may be related to the up-regulation of IRS-1,GluT4 expression in skeletal muscle.
【作者单位】： 甘肃医学院;和政县人民医院心内科;甘肃省人民医院心内科;
【基金】：甘肃省生卫生行业科研计划项目(GSWSKY-2015-04)
【分类号】：R589
，
本文编号：2522096
[Abstract]:Objective to observe the effect of inulin combined with swimming training on the expression of insulin receptor substrate 1 (IRS-1) and glucose transporter 4 (GluT4) in skeletal muscle of (MS) rats with metabolic syndrome. Methods MS rat model was established by high glucose, high fat and high salt feeding combined with intraabdominal injection of STZ. After 8 weeks of intervention, FBG,HbA_1c and FIns, were detected by HOMA-IR;HE staining, and the expression of IRS-1,GluT4 in skeletal muscle of rats was detected by immunoblotting and immunohistochemistry. Results compared with the normal diet control group, FBG,HbA_1c,FIns and HOMA-IR in the model group were increased (P01), (NAFLD) pathological changes were found in the liver, and the expression of IRS-1,GluT4 in skeletal muscle was decreased (P01). Compared with the model group, FBG,HbA_1c,FIns and HOMA-IR in the inulin combined swimming training group decreased (P 0.01), NAFLD) and the expression of IRS-1,GluT4 in skeletal muscle increased (P 0.01). Conclusion inulin combined swimming training can decrease the levels of FBG,HbA_1c and FIns and alleviate the pathological changes of NAFLD in MS rats. The mechanism of improving IR, may be related to the up-regulation of IRS-1,GluT4 expression in skeletal muscle.
【作者单位】： 甘肃医学院;和政县人民医院心内科;甘肃省人民医院心内科;
【基金】：甘肃省生卫生行业科研计划项目(GSWSKY-2015-04)
【分类号】：R589
，
本文编号：2522096
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