IGFBP2通过ERK1/2信号通路调控RA-FLS细胞生物学行为机制研究
发布时间：2020-12-20 00:25
目的:探讨胰岛素样生长因子结合蛋白2（Insulinlike growth factor binding protein2,IGFBP2）对类风湿关节炎成纤维样滑膜细胞（RA-FLS）生物学行为的作用,以及IGFBP2对细胞外调节蛋白激酶（Extracellular regulated protein kinases1/2,ERK1/2）的作用。方法:首先,我们原代培养类风湿关节炎成纤维样滑膜细胞（RA-FLS）和正常成纤维样滑膜细胞（FLS）,并采用q RT-PCR、Western blot及免疫荧光技术检测IGFBP2 m RNA和蛋白在FLS和RA-FLS中的表达水平。其次,RA-FLS细胞被转染si RNA-IGFBP2和si RNA-NC,q RT-PCR检测IGFBP2在RA-FLS细胞中的干扰效果;CCK-8实验检测IGFBP2敲低后对RA-FLS细胞增殖能力的影响;流式细胞仪检测IGFBP2敲低后对RA-FLS细胞凋亡的影响;Transwell检测IGFBP2敲低对RA-FLS细胞侵袭能力的影响;划痕实验检测IGFBP2敲低对RA-FLS细胞迁移能力的影响。此外,RA-...
【文章来源】：暨南大学广东省 211工程院校
【文章页数】：78 页
【学位级别】：博士
【部分图文】：
qRT-PCR检测IGFBP2mRNA在成纤维样滑膜细胞（FLS）和类风湿关节炎
3 Western blot检测IGFBP2蛋白在成纤维样滑膜细胞（FLS）和类风湿关节炎维样滑膜细胞(RA-FLS)中的表达水平。相对于FLS细胞，IGFBP2在RA-FLS中高表达。ure 3 Western blot assay was used to detect the expression level of IGFBP2 inoblast-like synoviosytes (FLS) and fibroblast-like synoviosytes from rheumatoidritis patients (RA-FLS). The expression level of IGFBP2 was high-expressed in-FLS cells compared with FLS cells.免疫荧光检测 IGFBP2 在 RA-FLS 中表达分布为了进一步确认 IGFBP2 在类风湿关节炎成纤维样滑膜细胞中表达，我们培养成纤维样滑膜细胞（FLS）和类风湿性关节炎成纤维样滑膜细胞-FLS)，免疫荧光检测 IGFBP2 蛋白在 RA-FLS 和 FLS 细胞中的表达分布情
FBP2 在类风湿关节炎成纤维样滑膜细胞中表达，主要分布在细-FLS 和 FLS 细胞，免疫荧光检测 IGFBP2 蛋白在 RA-FLS 和情况。左侧为细胞核 DAPI 着色，中间为 IGFBP2，右侧为 M色，×200 倍）4 IGFBP2 was highly expressed in fibroblast-like synoviosoid arthritis patients (RA-FLS). We cultured RA-FLS and FLS cexpression level of IGFBP2 was detected by the immunofluorescesuperimposed images of IGFBP2 in green and nuclei (DAPInofluorescence staining, ×200）-PCR 检测 IGFBP2 敲低后 mRNA 表达
【参考文献】：
期刊论文
[1]Expression of insulin-like growth factor binding protein-2 in gastric carcinoma and its relationship with cell proliferation[J]. Liang-Hui Shi, Guo-Hai Zhao, Ya-Bin Xia, Yi-Sheng Zhang, Department of Surgery, Affiliated Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui Province, China Xiao-Qun Zhu, Department of Pathology, Wannan Medical College, Wuhu 241001, Anhui Province, China. World Journal of Gastroenterology. 2006(39)
本文编号：2926848
【文章来源】：暨南大学广东省 211工程院校
【文章页数】：78 页
【学位级别】：博士
【部分图文】：
qRT-PCR检测IGFBP2mRNA在成纤维样滑膜细胞（FLS）和类风湿关节炎
3 Western blot检测IGFBP2蛋白在成纤维样滑膜细胞（FLS）和类风湿关节炎维样滑膜细胞(RA-FLS)中的表达水平。相对于FLS细胞，IGFBP2在RA-FLS中高表达。ure 3 Western blot assay was used to detect the expression level of IGFBP2 inoblast-like synoviosytes (FLS) and fibroblast-like synoviosytes from rheumatoidritis patients (RA-FLS). The expression level of IGFBP2 was high-expressed in-FLS cells compared with FLS cells.免疫荧光检测 IGFBP2 在 RA-FLS 中表达分布为了进一步确认 IGFBP2 在类风湿关节炎成纤维样滑膜细胞中表达，我们培养成纤维样滑膜细胞（FLS）和类风湿性关节炎成纤维样滑膜细胞-FLS)，免疫荧光检测 IGFBP2 蛋白在 RA-FLS 和 FLS 细胞中的表达分布情
FBP2 在类风湿关节炎成纤维样滑膜细胞中表达，主要分布在细-FLS 和 FLS 细胞，免疫荧光检测 IGFBP2 蛋白在 RA-FLS 和情况。左侧为细胞核 DAPI 着色，中间为 IGFBP2，右侧为 M色，×200 倍）4 IGFBP2 was highly expressed in fibroblast-like synoviosoid arthritis patients (RA-FLS). We cultured RA-FLS and FLS cexpression level of IGFBP2 was detected by the immunofluorescesuperimposed images of IGFBP2 in green and nuclei (DAPInofluorescence staining, ×200）-PCR 检测 IGFBP2 敲低后 mRNA 表达
【参考文献】：
期刊论文
[1]Expression of insulin-like growth factor binding protein-2 in gastric carcinoma and its relationship with cell proliferation[J]. Liang-Hui Shi, Guo-Hai Zhao, Ya-Bin Xia, Yi-Sheng Zhang, Department of Surgery, Affiliated Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui Province, China Xiao-Qun Zhu, Department of Pathology, Wannan Medical College, Wuhu 241001, Anhui Province, China. World Journal of Gastroenterology. 2006(39)
本文编号：2926848
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