SELL基因在中国汉族重型痤疮分子水平的研究

发布时间：2018-01-02 18:09

本文关键词：SELL基因在中国汉族重型痤疮分子水平的研究　出处：《昆明医科大学》2015年硕士论文　论文类型：学位论文

更多相关文章： SELL 重型痤疮 实时荧光定量PCR ELISA

【摘要】：研究背景前期研究利用GWAS方法对大量中国汉族重型痤疮样本进行研究,分析发现基因SELL是重型痤疮的易感基因。但SELL基因在分子水平与重型痤疮的相关性未见报道,本课题就这一方向进行研究。目的探讨SELL基因在重型痤疮及健康对照中分子水平上的表达差异。方法1)收集通过Pillsbury分类法收集的86例中国汉族重型痤疮及100例健康对照作为研究对象,其临床资料通过以调查问卷的形式并且填写统一设计的重型痤疮调查表和健康对照调查表获得。2)逐一提取外周血白细胞的总RNA,通过实时定量PCR方法检测患者外周血白细胞中SELL的mRNA表达水平,以β-actin基因为内参基因用2(-ΔΔCT)法得到结果。3)逐一分离外周血清,应用ELISA方法对重型痤疮和健康对照血清中可溶性L-选择素的浓度进行检测。4)所得数据用独立样本t检验、卡方检验、二分类Logistic回归分析方法分析。结果1)重型痤疮和健康对照L-选择素mRNA逆转录表达水平有差异,且差异有统计学意义(β=0.534, OR=0.238, P0.05,95%CI= 1.07-2.27)。2)重型痤疮和健康对照血清可溶性L-选择素差异有统计学意义(β=0.05,OR=0.22,P0.05,95%CI=1.01-1.10)。结论基因SELL mRNA逆转录及血清可溶性L-选择素的浓度在重型痤疮中表达水平较健康对照升高,提示重型痤疮中基因SELL在转录及翻译水平表达上调,揭示基因SELL在重型痤疮分子水平上表达上调,基因SELL与重型痤疮在分子表达上具有相关性。
[Abstract]:On the background of previous research using GWAS method to study a large number of Han China severe acne sample analysis found that the gene SELL is susceptible to severe acne. But the gene SELL gene and severe acne at the molecular level the correlation has not been reported, the research on this direction. Objective: To investigate SELL gene in severe acne and healthy control expression in the molecular level. Methods 1) collected by Pillsbury classification method to collect 86 cases of Han Chinese severe acne and 100 healthy volunteers as the research object, the clinical data by completing the unified design of the questionnaire and the health survey of severe acne control.2 in the form of a questionnaire) and one by one to extract total RNA of peripheral blood the cells of the SELL by real-time quantitative PCR method for detection of peripheral blood cell of patients with mRNA in the expression levels of reference genes with 2 beta -actin gene (delta delta CT) The results of.3 method) one by one from the peripheral serum, using ELISA method of acne and healthy control serum soluble L- selectin concentrations were detected.4 heavy) data obtained using independent samples t test, chi square test and analysis method of two classification Logistic regression. Results 1) acne and healthy control L- selectin mRNA reverse transcriptase the expression difference of heavy, and the difference was statistically significant (P =0.534, OR=0.238, P0.05,95%CI=, 1.07-2.27).2) acne and healthy control serum soluble L- selectin significantly heavy (beta =0.05, OR= 0.22, P0.05,95%CI=1.01-1.10). Conclusion SELL gene reverse transcription mRNA and serum soluble L- selectin levels increased compared with healthy controls the expression in severe acne, suggesting that upregulation of the expression of SELL gene in severe acne in the level of transcription and translation, revealing the gene SELL at the molecular level on the expression of severe acne Modulation, gene SELL and severe acne are associated with molecular expression.

【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R758.733

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