2940nm Er:YAG激光联合儿茶素抗鼠皮肤光损伤作用的实验研究

发布时间：2018-01-09 20:39

本文关键词：2940nm Er:YAG激光联合儿茶素抗鼠皮肤光损伤作用的实验研究　出处：《临床皮肤科杂志》2015年06期 　论文类型：期刊论文

【摘要】：目的:探究2 940 nm Er:YAG激光、儿茶素抗光损伤的作用,进一步探讨两者对于光老化皮肤氧化应激的可能作用机制。方法:将60只昆明小鼠分为5组:A组(对照组)、B组(光老化组)、C组(光老化+激光组)、D组(光老化+儿茶素组)、E组(光老化+激光+儿茶素组),每组各12只,除对照组外,对其他组小鼠分别行持续8周紫外线照射、持续4周儿茶素、激光处理再观察4周后,对各组小鼠皮肤结构、超氧化物岐化酶(SOD)活力、丙二醛(MDA)含量进行检测。结果:B组小鼠皮肤肉眼可见皮肤干燥、粗糙、皮沟明显、色素沉着明显;苏木精-伊红(HE)染色显示小鼠皮肤表皮层增厚厚度不均一,角质层增生偶可见脱落;真皮层胶原减少,厚度变薄,纤维层断裂,胶原纤维变粗条索化,腺体增生;与其余各组小鼠相比SOD活力明显降低(P0.01),MDA含量明显增多(P0.01)。与B组相比较,其他各干预组小鼠皮肤均有明显改善,皮肤较光滑、红润、光泽度好、细腻、动态性皱纹基本消失、无明显色素沉着;HE染色可见表皮层结构完整,但仍可见角质层脱落,真皮层变厚,可见新生胶原,腺体稍增多,排列较均匀;SOD活力明显升高(P0.01);MDA含量明显下降,差异均具有统计学意义(P0.01)。E组与C组相较SOD活力值、MDA含量差异均有统计学意义(P0.01)。结论:2 940 nm Er:YAG激光、儿茶素均可促进皮肤紧致、减缓光老化、刺激胶原再生、增强SOD活性、降低MDA含量,两者联合应用较单独使用激光疗效更佳,值得进一步深入研究,为临床治疗和研究提供可靠依据。
[Abstract]:Objective: to investigate the effect of catechin on light damage induced by 2 940 nm Er:YAG laser. Methods: 60 Kunming mice were divided into 5 groups: group A (control group) and group B (photoaging group). Group C (photoaging laser group), group D (photoaging catechin group), group E (photoaging laser catechin group, 12 rats in each group, except control group). The mice in other groups were exposed to ultraviolet radiation for 8 weeks and catechin for 4 weeks. After 4 weeks of laser treatment, the skin structure and SOD activity of each group were observed. The content of malondialdehyde (MDA) was detected. Results in group B, the skin was dry, rough, furrowed and pigmented. Hematoxylin-eosin (HEH) staining showed that the thickness of the skin epidermis was not uniform and the keratinocytes were occasionally exfoliated. The cortical collagen decreased, the thickness became thinner, the fibrous layer was broken, the collagen fibers became thicker and striped, and the glands proliferated. Compared with the other groups, the activity of SOD decreased significantly, the content of SOD increased significantly. Compared with group B, the skin of other intervention groups were significantly improved. The skin is smooth, ruddy, glossy, delicate, dynamic wrinkles disappear basically, no obvious pigmentation; The structure of the epidermis was intact in HE staining, but the cuticular layer was shedding, the dermis became thicker, the new collagen was visible, the glands increased slightly, and the arrangement was even. The activity of SOD increased significantly (P 0.01). The content of MDA decreased significantly, and the difference was statistically significant (P 0.01). Group E was significantly lower than group C (P 0.01) than group C (P < 0.05). Conclusion 2 940nm Er:YAG laser can promote skin compaction, slow photoaging and stimulate collagen regeneration. Enhancing the activity of SOD and reducing the content of MDA, the combined use of the two methods is more effective than laser alone, which is worthy of further study and provides a reliable basis for clinical treatment and research.
【作者单位】：南昌大学附属眼科医院整形美容科;温州和平整形医院整形外科;
【分类号】：R751.05
【正文快照】： 皮肤老化是一个自然老化与光老化伴行的复杂过程,自然老化一般指伴随着其他器官功能退化的同时所发生的皮肤结构、功能、代谢的改变;光老化主要集中在身体暴露部位,由紫外线照射引起,主要表现为皮肤皱纹较深、皮肤松弛、呈皮革样、色素斑增多以及血管扩张;组织学检查大多可见

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