百草益肤制剂对小鼠变应性接触性皮炎的影响及机制研究

发布时间：2018-02-12 07:21

本文关键词： 变应性接触性皮炎百草益肤制剂 IL-2 IFN-γ　出处：《扬州大学》2012年硕士论文　论文类型：学位论文

【摘要】：变应性接触性皮炎(Allergic contact dermatitis,ACD)是皮肤或黏膜接触致敏物后主要由T细胞介导的迟发型变态反应引起的炎症性皮肽病,包括来源于T细胞等的多种细胞因子、趋化因子以及共刺激分子参与了整个炎症过程。目前大多数观点认为ACD是由分泌IL-2、IFN-γ的Thl细胞介导的,其发生是因为Thl/Th2分化失衡使其免疫紊乱。ACD的治疗以去除致敏原,外用糖皮质激素,应用保湿剂等为主。糖皮质激素应用是目前控制急性期变态反应及炎症的主要方法,但长期应用会造成肾上腺皮质功能低下、继发感染等全身副作用,还可引起皮肽萎缩、毛细血管扩张、激素性白内障、青光眼、颜面红斑等,从而影响儿童的生长发育和成人内分泌系统,停药后易复发,久用产生治疗抵抗的现象也普遍存在。因此,联合应用其它治疗方法,寻找副作用小,并且高效的治疗变应性接触性皮炎的制剂,尽可能减少糖皮质激素用量,甚至停用,是目前临床治疗努力的方向。目前,我们运用现代制剂学方法自制的亲诺婴儿百草益肤制剂以苦参、库拉索芦荟凝胶等为主要原料,其中苦参中的苦参碱可对抗急性渗出性炎症,具有非甾体抗炎的特性,苦参碱还具有免疫抑制作用,分子机制研究表明苦参碱对P物质诱导单核细胞趋化蛋白-1(MCP-1)具有干预作用；库拉索芦荟含较多的黏液素,其中多糖类物质含量高,它不仅具有免疫调节功能,还有助于细胞分裂,对皮肤的保养修复可起重要作用。本制剂具有清热解毒、燥湿、收敛止痒等特点,对湿疹皮炎具有疗效好、使用方便、无明显副作用等优点。本研究通过建立小鼠变应性接触性皮炎模型,来探讨本制剂对其的影响及可能机制。目的通过建立小鼠变应性接触性皮炎模型,观察百草益肤制剂对其的影响并探讨可能机制。方法本文从实验角度,以2,4—二硝基氟苯(DNFB)诱发建立小鼠ACD模型,外用亲诺婴儿百草益肤制剂及外用婴儿湿疹膏、丁酸氢化可的松乳膏作对照,观察分组给药后小鼠耳朵的肿胀度及组织病理变化,同时用ELISA及免疫组织化学方法分别检测不同时相各组小鼠血清及耳上皮组织中白介素-2及干扰素-γ表达水平。结果亲诺婴儿百草益肽膏、亲诺婴儿百草益肤水、丁酸氢化可的松乳膏及婴儿湿疹膏组均能降低小鼠左耳肿度,下调小鼠血清及皮损中白介素-2及干扰素-Y的表达,且亲诺婴儿百草益肤膏与丁酸氢化可的松乳膏组疗效无明显差别(P0.05),与亲诺婴儿百草益肤水及婴儿湿疹膏组比较,差异有统计学意义(P0.05)。结论百草益肽制剂对小鼠变应性接触性皮炎有抑制作用,其作用机制可能与抑制白介素-2及干扰素-γ等细胞因子的释放有关。意义本研究为该药的开发及临床应用提供了实验依据,从而印证该药治疗ACD及此类疾病如湿疹等的可行性,对解除患者痛苦,提高患者生活质量,充分发挥中医中药的优势具有重要意义。
[Abstract]:Allergic contact dermatitis (Allergic contact, dermatitis, ACD) is caused by delayed hypersensitivity skin or mucosal contact allergens was mainly mediated by T cells in the inflammatory skin disease, including a variety of cytokines from T cells, chemokines and costimulatory molecules involved in the inflammatory process at present the majority view ACD is secreted by IL-2 cells, Thl IFN- gamma guide, it occurs when the Thl/Th2 imbalance in the treatment of the immune disorder.ACD to remove allergens, topical corticosteroids, application of moisturizing agent. Glucocorticoid application is the main method to control allergy and the acute phase of inflammation, but long-term use will cause adrenal insufficiency, systemic side effects of secondary infection, can also cause skin atrophy, telangiectasia, steroid induced cataract, glaucoma, facial red spots, thus. Sound growth and endocrine system of adult children, easy to relapse after drug withdrawal, long produced treatment resistance phenomenon is also widespread. Therefore, combined with other treatments for side effects, preparation and efficient treatment of allergic contact dermatitis, reducing dosage of glucocorticoid and even disabled is the clinical treatment direction.
At present, we made use of modern preparation methodology Pro Nobel herbal preparations for the baby skin of Sophora flavescens, Kuraso aloe gel as the main raw materials, wherein the Matrine in matrine against acute exudative inflammation, has the characteristics of non steroidal anti-inflammatory, matrine has immunosuppressive effect, the molecular mechanism study indicated that matrine on substance P induced monocyte chemoattractant protein -1 (MCP-1) with intervention; Kuraso aloe contains more mucus, the polysaccharide content is high, it not only has the immunoregulatory function, also contributes to cell division, which plays an important role in the repair and maintenance of the skin. The preparation has heat clearing and detoxifying, removing dampness. The convergence characteristics of dermatitis and eczema itching, has good curative effect, convenient use and has no obvious side effects. In this study we established a mouse model of allergic contact dermatitis, to investigate the effect of the preparation of the And possible mechanisms.
objective
Through the establishment of a model of allergic contact dermatitis in mice, the effect of a hundred herbs and beneficial skin preparation on the dermatitis was observed and the possible mechanism was discussed.
Method
This article from the experimental point of view, the 2,4 - two 4-dinitrofluorobenzene (DNFB) induced mice ACD model, topical herbal preparation Pro Nobel baby skin and topical baby eczema cream, Hydrocortisone Cream butyric acid group, observation group after administration of the swelling of mouse ear and pathological changes, the expression level at the same time with ELISA and immunohistochemistry methods were detected by the mice serum and ear epithelial tissues of interleukin -2 and interferon gamma.
Result
Pro Nobel baby herbal peptide cream, pro Nobel herbal baby lotion, baby eczema cream and Hydrocortisone Cream butyric acid group could reduce the mice left ear swelling, down regulated expression of interleukin in serum and skin lesions in mice -2 and interferon -Y, and pro Nobel baby herbal skin cream and butyric acid groups had no significant difference (Hydrocortisone Cream P0.05), and pro Nobel baby herbal toner and baby eczema cream group, the difference was statistically significant (P0.05).
conclusion
Paraquat peptide preparation can inhibit allergic contact dermatitis in mice, and its mechanism may be related to the release of cytokines such as interleukin -2 and interferon gamma.
Significance
This study provides experimental evidence for the development and clinical application of the drug, so as to confirm the feasibility of the drug in the treatment of ACD and other diseases such as eczema. It is important for relieving the pain of patients, improving the quality of life of patients and giving full play to the advantages of traditional Chinese Medicine.

【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R758.22;R965

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