银屑病素在T细胞分泌IL-8中的作用

发布时间：2018-02-13 01:25

本文关键词： 银屑病 Psoriasin T细胞 IL-　出处：《中国皮肤性病学杂志》2014年04期 　论文类型：期刊论文

【摘要】：目的研究抗菌蛋白银屑病素(psoriasin)在T细胞分泌IL-8中的作用。方法抽取正常人和银屑病患者外周血,提取T淋巴细胞,用ELISA的方法检测正常人和银屑病患者外周血中IL-8的表达变化。结果未受psoriasin刺激的正常人血T细胞不能产生IL-8,而在psoriasin刺激2,4,8和12h后,可见IL-8的表达升高,且有时间和剂量依赖性,8h时,产生IL-8的量最多,可达492pg/mL;未受psoriasin刺激的银屑病患者血T细胞可产生少量IL-8,银屑病患者外周血在psoriasin刺激2,4,8和12h后,也见IL-8的表达升高,4h时的产生量已经相当于正常细胞8h的产生量,且8h时产生量最多,可达1 007.5pg/mL。结论 Psoriasin刺激银屑病患者外周血T细胞产生IL-8的速度比正常人更快,剂量更多。
[Abstract]:Objective to study the role of antifungal protein psoriasis in the secretion of IL-8 by T cells. Methods T lymphocytes were extracted from the peripheral blood of normal subjects and patients with psoriasis. ELISA was used to detect the expression of IL-8 in the peripheral blood of normal subjects and psoriatic patients. Results the T cells without psoriasin stimulation could not produce IL-8, but the expression of IL-8 increased after psoriasin stimulation for 2h and 12h. The amount of IL-8 produced was the highest (492pg / mL) at 8h in a time-and dose-dependent manner, and a small amount of IL-8 was produced in the blood T cells of psoriatic patients without psoriasin stimulation, and the peripheral blood of psoriatic patients was stimulated by psoriasin for 2h and 12h. It was also found that the expression of IL-8 increased at 4 h, and the amount of IL-8 produced was the same as that of normal cells at 8 h, and at 8 h it was the highest, up to 1 007.5 PG / mL. Conclusion Psoriasin stimulates the production of IL-8 in peripheral blood T cells of psoriatic patients more rapidly than that of normal controls.
【作者单位】：西安交通大学医学院第二附属医院皮肤科;西安交通大学医学院;西安市第一医院皮肤科;
【基金】：国家自然科学基金资助项目(81071299,81371732) 教育部新世纪优秀人才支持计划中央高校基本科研业务费交大二院重点基金资助
【分类号】：R758.63

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