纳米金结合恩度抑制小鼠黑色素瘤肺转移

发布时间：2018-02-16 18:55

本文关键词： 纳米金恩度黑色素瘤肿瘤转移上皮-间充质转化　出处：《中国病理生理杂志》2017年08期 　论文类型：期刊论文

【摘要】：目的:观察纳米金结合恩度对小鼠黑色素瘤肺转移的影响并探讨其机制。方法:C57BL/6小鼠24只,构建小鼠黑色素瘤B16-F10细胞自发性肺转移模型,随机分为恩度组、纳米金组、纳米金结合恩度组和模型组。成瘤后各组小鼠均经尾静脉注射相应药物0.1 mL(每天1次)。9 d后麻醉小鼠并切取原位肿瘤,继续饲养2周后处死小鼠取肺组织观察转移情况。HE染色观察原位肿瘤坏死情况,免疫染色检测肿瘤CD31、碳酸酐酶IX(CA-IX)、vimentin和闭锁小带蛋白1(ZO-1)的表达。结果:与模型组相比,药物处理组小鼠的肺转移灶均明显减少,其中纳米金结合恩度组的肺转移抑制率最高,肿瘤坏死明显减少,肿瘤中CD31、CA-IX和vimentin的表达量显著降低,ZO-1的表达量显著升高。结论:与单用恩度或纳米金相比,纳米金结合恩度可明显抑制小鼠黑色素瘤肺转移,其机制可能为使肿瘤新生血管减少及血管正常化,改善肿瘤缺氧,从而抑制肿瘤上皮-间充质转化,使肿瘤细胞间紧密连接增加而侵袭性减低。
[Abstract]:Objective: to observe the effect of nano-gold combined with Endol on lung metastasis of melanoma in mice and explore its mechanism. Methods: Twenty-four mice with B16-F10 melanoma were randomly divided into two groups: Endol group and nano-gold group. After tumorigenesis, the mice in each group were injected with the corresponding drug 0.1 mL via caudal vein (once a day for 9 days, then anesthetized mice were anesthetized and the tumor in situ was removed. After 2 weeks of feeding, the mice were sacrificed to observe metastasis. He staining was used to observe tumor necrosis in situ. The expression of CD31, carbonic anhydrase IXCA-IXVIMIMN and atresia band protein 1 (ZO-1) were detected by immunostaining. Results: compared with the model group, the expression of CD31, IXCCA-IXVIMN and atresia zone protein 1 (ZO-1) were detected by immunohistochemical staining. In the drug treatment group, the lung metastasis was significantly decreased, and the inhibition rate of lung metastasis was the highest and the tumor necrosis was significantly decreased in the nano-gold combined with Endol group. The expression of CD31, CA-IX and vimentin significantly decreased the expression of ZO-1 in tumor. Conclusion: compared with mono-degree or nano-gold, nano-gold binding Endox can significantly inhibit lung metastasis of melanoma in mice. The mechanism may be to reduce and normalize tumor neovascularization, improve tumor anoxia, inhibit epithelial-mesenchymal transformation, increase tight junctions between tumor cells and decrease invasiveness.
【作者单位】：暨南大学附属第一医院胃肠外科;
【基金】：国家自然科学基金资助项目(No.81472849) 广东省自然科学基金资助项目(No.2014A030313383;No.2014A030313382)
【分类号】：R739.5

【相似文献】