维生素C对紫外线诱导的光老化大鼠皮肤结构的影响

发布时间：2018-02-20 01:17

本文关键词： 维生素C 抗光老化皮肤结构模型大鼠　出处：《中国比较医学杂志》2015年06期 　论文类型：期刊论文

【摘要】：目的研究维生素C对光老化大鼠皮肤结构的保护作用,为临床用药和保健提供依据。方法用紫外线辐射背部皮肤的方法建立光老化大鼠模型,每周照射2次,连续4周,UVA累计辐射量为123 J/cm2,UVB累计辐射量为5 J/cm2。造模期间,大鼠每天灌胃给予维生素C溶液50 mg/kg,持续30 d。实验结束后,比较模型组和治疗组照射部位皮肤在大体形态、光镜和透射电镜下的组织结构差异。结果与模型组相比,维生素C组以下方面的皮肤结构损伤程度得到减轻:红疹、溃疡、表皮细胞增生、表皮增厚、真皮血管扩张、炎细胞浸润、成纤维细胞增生、内质网扩张、线粒体肿胀和空泡化、弹力纤维变性和局灶性蓄积、胶原纤维横断面粗细不均。结论大鼠皮肤光老化模型建立成功。维生素C对紫外线诱导的光老化皮肤结构损伤有明显的保护作用。
[Abstract]:Objective to study the protective effect of vitamin C on the skin structure of photoaged rats, and to provide evidence for clinical use and health care. Methods the model of photoaging rats was established by ultraviolet radiation on the back skin, which was irradiated twice a week. The cumulative radiation dose of UVA was 123J / cm ~ 2 路min ~ (-2) for 4 weeks. During the modeling period, rats were given 50 mg / kg vitamin C solution daily for 30 days. After the experiment, the skin of the model group and the treatment group were compared. Results compared with the model group, the degree of skin structure damage in vitamin C group was alleviated in the following aspects: rash, ulcer, epidermal cell proliferation, epidermis thickening, dermis vasodilation. Inflammatory cell infiltration, fibroblast proliferation, endoplasmic reticulum dilatation, mitochondrial swelling and vacuolation, elastic fiber degeneration and focal accumulation, Conclusion the photoaging model of rat skin was established successfully. Vitamin C has obvious protective effect on ultraviolet induced photoaging skin structure damage.
【作者单位】：广东省医学实验动物中心比较医学实验室;无限极(中国)有限公司;
【基金】：国家自然科学基金(编号:81403212) 广东省医学科研基金(编号:B2014062) 广东省中医药管理局基金(编号:20142019)
【分类号】：R758.1

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