梅毒患者驱梅治疗前、后外周血PBMC Toll样受体2表达

发布时间：2018-02-22 23:36

本文关键词： 梅毒 Toll样受体流式细胞术　出处：《中国皮肤性病学杂志》2015年10期 　论文类型：期刊论文

【摘要】：目的观察梅毒患者驱梅治疗前、后外周血单一核细胞(PBMC)表面Toll样受体2的变化情况。方法对初诊为梅毒的患者分别于驱梅治疗前及驱梅治疗结束后1年抽取外周静脉血检测其PBMC的TLR2+CD14+表达情况及TLR2+CD14+占CD14+细胞群的比例。并选60例健康者作为对照组进行比较。结果初诊时梅毒患者外周血PBMC的中CD14+TLR2+表达及百分比均明显低于正常对照组,且差异均有统计学意义(P0.001)。其中一期的初诊梅毒患者外周血PBMC的TLR2+CD14+阳性率低于二期初诊梅毒患者和隐性梅毒患者,差异有统计学意义(P0.001);血清固定组复诊时TLR2(MFI)表达低于血清阴转组TLR2(MFI)表达(P0.001),血清固定组的初诊和复诊者TLR2+CD14+细胞(%)均低于血清阴转组TLR2+CD14+细胞(%)(P0.05)。结论 TLR2+CD14+阳性细胞表达上调不够,其可能导致发生梅毒血清固定。
[Abstract]:Objective to observe the syphilis patients before treatment, Methods the changes of Toll-like receptor 2 on the surface of peripheral blood mononuclear cells (PBMCs) were observed in patients with newly diagnosed syphilis. TLR2 CD14 expression of PBMC was detected in peripheral venous blood samples from patients with syphilis before treatment and 1 year after treatment. The percentage of TLR2 CD14 in CD14 cell group and 60 healthy controls were compared. Results the expression and percentage of CD14 TLR2 in peripheral blood of syphilis patients were significantly lower than those in normal control group. The positive rate of TLR2 CD14 in peripheral blood of primary syphilis patients was lower than that of secondary syphilis patients and recessive syphilis patients. The difference was statistically significant (P 0.001), the expression of TLR2mF-MFI in serum fixation group was lower than that in sero-negative transition group (P 0.001), and the expression of TLR2 CD14 cells in serum fixation group was lower than that in TLR2 CD14 cell line P0.050.Conclusion the expression of TLR2 CD14 positive cells is not enough, and the expression of TLR2 CD14 positive cells is not enough. It may lead to syphilis serum fixation.
【作者单位】：宁波大学医学院附属医院皮肤科;宁波市李惠利医院检验科;
【基金】：宁波大学校级基金(xyy12006)
【分类号】：R759.1

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