AQP5在手汗症合并腋窝多汗患者腋窝汗腺中的表达及意义

发布时间：2018-02-25 02:14

本文关键词： AQP5 手汗症腋窝多汗 HE染色免疫组织化学　出处：《福建医科大学》2010年硕士论文　论文类型：学位论文

【摘要】： 目的:检测手汗症伴腋窝多汗患者腋窝汗腺中水通道蛋白(aquaporin,AQP)的表达并探讨其与手汗症伴腋窝多汗发病机制的关系。方法:收集34例手汗症伴腋窝多汗患者腋窝汗腺标本,按Lai等[1]的手汗分级标准:其中重度多汗32例,中度多汗2例;另取正常非手汗伴腋窝多汗患者腋窝汗腺标本8例作对照组。应用HE染色法对腋窝汗腺进行染色,在光镜下分别计算其腋窝汗腺数目,并进行比较分析;应用免疫组织化学法(SP法)测定水通道蛋白(AQP1,AQP3和AQP5)的表达水平并进行比较分析。结果:HE染色结果显示:光镜下可见汗腺的分泌部和导管部,汗腺细胞胞核被染成深蓝色,胞质被染成红色;有些患者的汗腺呈团或呈簇聚集在一起,有些患者的汗腺是单个独立分布。汗腺计数结果显示:手汗症伴腋窝多汗患者腋窝汗腺数目较对照组无明显统计学差异(Z=-1.506, P=0.132)。免疫组化结果显示:两组腋窝汗腺上均有水通道蛋白AQP5的表达,均无AQP1,AQP3表达。分析比较显示水通道蛋白AQP5在手汗伴腋窝多汗患者腋窝汗腺中表达较对照组显著升高(Z=-3.523, P㩳0.001)。结论:两组腋窝汗腺的数目是没有明显统计学差异的。水通道蛋白AQP1,AQP3不参与腋窝汗腺的分泌,AQP5与手汗症伴腋窝多汗患者腋窝多汗有关,AQP5表达增强很可能是手汗症伴腋窝多汗患者腋窝多汗的发病机制之一。
[Abstract]:Aim: to detect the expression of aquaporin aquaporin (AQP) in the axillary sweat gland of the patients with palmar hyperhidrosis and to explore the relationship between aquaporin aquaporin and the pathogenesis of hyperhidrosis in the armpit. Methods: according to Lai's criteria, 32 cases were severe hyperhidrosis and 2 cases moderate hyperhidrosis. The axillary sweat glands were stained with HE staining method, and the number of axillary sweat glands were calculated under light microscope, and the number of axillary sweat glands were compared and analyzed. The expression levels of aquaporin-1 AQP3 and AQP5) were determined by immunohistochemical method (SP method) and compared with each other. Results: under light microscope, the secretory and ductal parts of sweat glands could be seen. The nuclei of sweat gland cells were stained dark blue and the cytoplasm was stained red. The sweat glands of some patients were clustered or clustered together. The number of axillary sweat glands in patients with palmar hyperhidrosis and hyperhidrosis was not significantly different from that in the control group. The immunohistochemical results showed that the number of axillary sweat glands in the two groups was higher than that in the control group. The expression of aquaporin AQP5 was observed. Analysis and comparison showed that the expression of aquaporin AQP5 in the sweat gland of hand sweat patients with axillary hyperhidrosis was significantly higher than that of the control group, and the expression of aquaporin AQP5 was significantly higher than that of the control group. 0.001. Conclusion: there is no significant difference in the number of axillary sweat glands between the two groups. Aquaporin AQP1- AQP3 does not participate in the secretion of axillary sweat glands. The increased expression of AQP5 may be related to the hyperhidrosis of the armpit in patients with palmar hyperhidrosis and hyperhidrosis. One of the pathogenesis of axillary hyperhidrosis in patients with axillary hyperhidrosis.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R758.7

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