促红细胞生成素对不同化疗药抗黑色素瘤活性的调节作用

发布时间：2018-02-25 06:25

本文关键词： 促红细胞生成素促红细胞生成素受体黑色素瘤达卡巴嗪顺铂　出处：《中华肿瘤防治杂志》2014年08期 　论文类型：期刊论文

【摘要】：目的:检测小鼠黑色素瘤细胞株B16促红细胞生成素受体(erythropoietin receptor,EPOR)的表达,探讨促红细胞生成素(erythropoietin,EPO)对达卡巴嗪和顺铂抗B16细胞活性的影响及与Bcl-2家族蛋白表达的关系。方法:采用RT-PCR和细胞免疫化学染色法检测B16细胞EPOR mRNA和蛋白的表达;MTT法和克隆形成试验检测EPO与达卡巴嗪或顺铂联合应用时对B16细胞增殖的影响;实时荧光定量RT-PCR和蛋白质印迹法检测EPO对B16细胞Bcl-2、Bcl-xL、Bax mRNA和蛋白表达的影响。结果:B16细胞表达EPOR mRNA和蛋白。EPO能有效减少达卡巴嗪诱导B16细胞的凋亡(P0.05),但未能显著影响顺铂对B16细胞的作用,P0.05。B16细胞经EPO处理后,其Bcl-2、Bcl-xL mRNA和蛋白表达水平明显增加(P0.05),Bax mRNA和蛋白表达水平未见明显变化,P0.05。结论:B16细胞表达EPOR,EPO可调节化疗药对B16细胞的抗肿瘤活性,且该作用与化疗药的类型有关,其作用机制可能与EPO影响Bcl-2家族蛋白表达有关。
[Abstract]:Objective: to detect the expression of erythropoietin receptor erythropoietin receptor (EPOR) in mouse melanoma cell line B16. To investigate the effect of erythropoietin (EPO) on the activity of dacarbazide and cisplatin against B16 cells and its relationship with the expression of Bcl-2 family proteins. Methods: the expression of EPOR mRNA and protein in B16 cells was detected by RT-PCR and immunocytochemical staining. The effects of EPO combined with dacarbazide or cisplatin on the proliferation of B16 cells were detected by PCR and Clonogenic Assay. Real-time fluorescence quantitative RT-PCR and Western blot were used to detect the effect of EPO on the expression of EPOR mRNA and protein in B16 cells. Results the expression of EPOR mRNA and protein. EPO could effectively reduce the apoptosis of B16 cells induced by Dhabarazine-induced apoptosis (P0.05N), but had no significant effect on the expression of B16 cells. The effect of cisplatin on B16 cell line P0.05.B16 cells were treated with EPO. The expression of Bcl-xL mRNA and Bcl-xL protein in B16 cells was significantly increased. There was no significant change in the expression of mRNA and protein in B16 cells. Conclusion the expression of EPORX EPO in B16 cells can regulate the antitumor activity of chemotherapeutic agents on B16 cells, and this effect is related to the type of chemotherapeutic agents. The mechanism may be related to the effect of EPO on the expression of Bcl-2 family proteins.
【作者单位】：河北医科大学第一医院检验科;河北医科大学第四医院肿瘤内科;河北医科大学第四医院科研中心;
【基金】：河北省卫生厅重点科技研究计划(20120289)
【分类号】：R739.5

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