腺病毒介导的SOCS1对模拟银屑病小鼠模型的影响

发布时间：2018-02-26 12:27

本文关键词： 银屑病细胞因子信号抑制因子腺病毒　出处：《华中科技大学学报(医学版)》2014年02期 　论文类型：期刊论文

【摘要】：目的探讨以腺病毒为载体介导的细胞因子信号抑制因子1(SOCS1)对模拟银屑病小鼠模型(小鼠尾部鳞片表皮模型)的影响。方法制备重组腺病毒Ad-SOCS1、Ad-sh-SOCS1和Ad-EGFP,用Western blot或荧光显微镜分析或观察这些病毒感染的小鼠肺上皮细胞(TC-1细胞)的蛋白表达。用ELISA试剂盒检测上述病毒感染TC-1细胞后的细胞培养液和小鼠血清中的IL-6和TNF-α的浓度。观察腺病毒介导的SOCS1表达对小鼠尾部鳞片表皮模型的病理变化的影响。结果成功构建的Ad-SOCS1、Ad-sh-SOCS1和Ad-EGFP能感染TC-1细胞并能有效表达各自的基因。与Ad-sh-SOCS1和Ad-EGFP组比较,Ad-SOCS1能显著抑制小鼠血清和IFN-γ诱导培养TC-1细胞后培养液中IL-6和TNF-α的水平(均P0.01),且Ad-SOCS1能显著增加小鼠尾部鳞状上皮颗粒层的形成。结论腺病毒介导的SOCS1对小鼠尾部表皮细胞增生和角化不全有抑制作用,其机制可能与抑制前炎症因子表达、促进颗粒细胞层的形成和抑制表皮细胞的增殖有关。
[Abstract]:Objective to investigate the effect of adenovirus mediated cytokine signal suppressor 1 (SOCS1) on mouse model of psoriasis (mouse tail scale epidermis model). Methods Recombinant adenovirus Ad-SOCS1 Ad-sh-SOCS1 and Ad-EGFP1 were prepared and detected by Western blot or fluorescence. The expression of IL-6 and TNF- 伪 in the culture medium and serum of mice infected with these viruses were detected by ELISA kit. The effect of adenovirus mediated SOCS1 expression on the pathological changes of mouse tail scale epidermis model. Results Ad-SOCS1 and Ad-EGFP could infect TC-1 cells and express their genes effectively. Ad-SOCS1 could be significantly inhibited by Ad-SOCS1 as compared with Ad-sh-SOCS1 and Ad-EGFP groups. The levels of IL-6 and TNF- 伪 in mouse serum and IFN- 纬 induced culture medium of TC-1 cells were all P 0.01, and Ad-SOCS1 could significantly increase the formation of granular layer of mouse tail squamous epithelium. Conclusion Adenovirus-mediated SOCS1 can induce the proliferation of mouse tail epidermal cells and the expression of TNF- 伪. Hypokeratosis has an inhibitory effect, The mechanism may be related to inhibiting the expression of proinflammatory factors, promoting the formation of granulosa cell layer and inhibiting the proliferation of epidermal cells.
【作者单位】：深圳市光明新区人民医院皮肤科;深圳市光明新区中心医院药剂科;中山大学附属第三医院检验科;
【基金】：深圳市2011年科技计划资助项目(No.201103214)
【分类号】：R758.63

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