梅毒螺旋体大环内酯类抗生素耐药位点的监测

发布时间：2018-02-28 04:19

  本文关键词： 梅毒螺旋体 大环内酯类抗生素 点突变　出处：《实用医学杂志》2017年15期 　论文类型：期刊论文

【摘要】：目的调查湖南衡阳地区梅毒螺旋体23 rRNA基因上A2058G和A2059G突变现状。方法常规PCR扩增一期梅毒患者下疳样本中Tp pol A基因,筛选Tp DNA阳性样本;巢氏PCR扩增Tp 23r RNA基因,分别采用限制性内切酶MboⅡ和BsaⅠ酶切纯化的PCR产物。结果 43例下疳分泌物中筛选出39例Tp DNA阳性样本;39例样本全部检出A2058G突变,而未检出A2059G突变。结论湖南衡阳地区Tp流行株存在高频率23rRNA基因点突变,临床上在治疗梅毒时应谨慎使用大环内酯类抗生素。
[Abstract]:Objective to investigate the status of A2058G and A2059G mutations in 23 rRNA gene of Treponema pallidum in Hengyang, Hunan Province. Methods the TP pol A gene was amplified by routine PCR from the chancre samples of primary syphilis patients, and the positive samples of TP DNA were screened out, and the Tp23r RNA gene was amplified by nested PCR. The purified PCR products were digested by restriction endonuclease Mbo 鈪，

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