梓醇、左旋紫草素、丹皮酚调控角质形成细胞表达人β防御素-2的机理

发布时间：2018-03-01 16:18

本文关键词： 中药单体人β防御素- JNK信号通路银屑病　出处：《中国皮肤性病学杂志》2016年03期 　论文类型：期刊论文

【摘要】：目的探讨梓醇、左旋紫草素、丹皮酚通过影响JNK信号通路发挥调控HBD-2的作用。方法用IL-1β和TNF-α各100ng/m L的混合物刺激Ha Ca T细胞18h建立HBD-2高表达及JNK信号通路活化的细胞学体外模型,配制含合适浓度的梓醇、左旋紫草素、丹皮酚培养基干预细胞模型,并设阴性及阳性对照。用实时荧光定量PCR法检测各组中HBD-2 m RNA的表达;用ELISA法检测培养上清中HBD-2蛋白的表达;用Western blot法检测各组中JNK蛋白的表达。结果左旋紫草素、丹皮酚和JNK抑制剂(阳性对照药物)可以明显下调细胞学体外模型中HBD-2基因及蛋白的表达(P0.05),左旋紫草素与JNK抑制剂相当(P0.05),丹皮酚弱于JNK抑制剂(P0.05)。梓醇则明显上调细胞学体外模型中HBD-2基因及蛋白的表达(P0.05)。左旋紫草素、丹皮酚和JNK抑制剂可以明显下调P-JNK2蛋白的相对含量,梓醇对P-JNK2蛋白无抑制作用,反而上调其表达。结论紫草、丹皮可能通过抑制JNK2MAPK信号通路的活化,下调HBD-2的表达,从而发挥其治疗银屑病的作用。地黄治疗银屑病的机制尚需从其他方面继续探讨。
[Abstract]:Objective to study catalpol and levoshikonin. Methods Paeonol stimulated Ha Ca T cells with the mixture of IL-1 尾 and TNF- 伪 100 ng / mL each for 18 h to establish a cytological model of high HBD-2 expression and activation of JNK signaling pathway, and to prepare catalpol with appropriate concentration. The expression of HBD-2 m RNA was detected by real-time fluorescence quantitative PCR method, and the expression of HBD-2 protein in culture supernatant was detected by ELISA method. Western blot method was used to detect the expression of JNK protein in each group. Paeonol and JNK inhibitor (positive control drug) could significantly down-regulate the expression of HBD-2 gene and protein in cytological model in vitro. The expression of HBD-2 gene and protein was similar to that of JNK inhibitor. Paeonol was weaker than that of JNK inhibitor, and catalpol was significantly up-regulated. Expression of HBD-2 Gene and protein in Cytology Model in Vitro. Paeonol and JNK inhibitor could significantly down-regulate the relative content of P-JNK2 protein. Catalpol did not inhibit the expression of P-JNK2 protein, but up-regulated its expression. Conclusion Paeonol and JNK inhibitor may down-regulate the expression of HBD-2 by inhibiting the activation of JNK2MAPK signaling pathway. The mechanism of Rehmannia glutinosa in the treatment of psoriasis needs to be further explored from other aspects.
【作者单位】：北京市平谷区医院皮肤病与性病科;中日友好医院皮肤病与性病科;中日友好医院临床研究所;
【基金】：北京市自然科学基金(7112122)
【分类号】：R758.63

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