内蒙古蒙古族寻常型银屑病与HLA-Cw及DRB1位点相关性研究

发布时间：2018-03-01 19:45

本文关键词： 银屑病 HLA 蒙古族相关性研究　出处：《内蒙古医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的:通过分析内蒙古蒙古族寻常型银屑病患者HLA-Cw及DRB1等位基因基因型频率，研究蒙古族人群寻常型银屑病与HLA-Cw及DRB1等位基因的相关性，探讨寻常型银屑病的致病基因并为银屑病病因学研究提供理论依据。通过对蒙古族寻常型银屑病与HLA相关性研究来丰富银屑病易感基因图谱，为今后银屑病相关研究提供有价值资料，为银屑病基因水平的诊断、治疗、预后评估及相关研究奠定理论基础。方法:本实验研究选择无血缘关系、3代以上连续生活在内蒙古境内的蒙古族人群，提取血液细胞基因组DNA，利用序列特异性引物聚合酶链反应（PCR-SSP）对蒙古族寻常型银屑病患者81例及正常蒙古族100例的HLA-Cw及DRB1位点等位基因进行基因分型。根据HLA各等位基因在患者组和对照组之间频率分布，利用SPSS13.0统计软件进行χ2检验，分析两组间各等位基因频率差异。结果:共检出10个HLA-Cw座位等位基因，12个HLA-DRB1座位等位基因。对这些检出的等位基因根据银屑病组和正常人组基因频率分布差异进行统计学分析，得出有显著性差异的等位基因是：HLA-Cw*04，Cw*06，DRB1*04和DRB1*07等位基因。其中，银屑病组HLA-Cw*06，DRB1*07等位基因频率显著高于正常人组，HLA-Cw*04、DRB1*04等位基因频率显著低于正常人组（Pc＜0.05）。在发病年龄＜40岁银屑病及家族史阴性患者中HLA-Cw*06、DRB1*07等位基因频率显著高于正常人组，，而HLA-Cw*04、DRB1*04等位基因频率显著低于正常人组（Pc＜0.05）。在发病年龄≥40岁的银屑病及家族史阳性患者中只有HLA-Cw*06等位基因频率显著高于正常人组（Pc＜0.05）。HLA-Cw*04、DRB1*04基因频率的下降及Cw*06、DRB1*07基因频率的上升在无家族史银屑病患者间均有统计学意义（Pc＜0.05），而Cw*06的基因频率还在家族史阳性患者中有显著上升（Pc＜0.05）。结论: HLA-Cw*06、DRB1*07等位基因可能是内蒙古地区蒙古族人群寻常型银屑病的易感基因。HLA-Cw*04、DRB1*04等位基因等位基因可能是内蒙古地区蒙古族人群寻常型银屑病发病的保护因子。HLA-DRB1*07等位基因可能是发病年龄＜40岁的银屑病的易感基因，而HLA-Cw*04、DRB1*04等位基因则可能是发病年龄＜40岁银屑病的保护因子。有银屑病家族史与无银屑病家族史在基因遗传上有一定差别，而HLA-Cw*06等位基因可能是二者共同的遗传基础。
[Abstract]:Objective: through the analysis of Inner Mongolia Mongolian patients with psoriasis vulgaris and HLA-Cw DRB1 allele genotype frequency correlation of Mongolian people with psoriasis vulgaris HLA-Cw and DRB1 alleles, pathogenic genes of psoriasis vulgaris and to provide a theoretical basis for psoriasis etiology research. To enrich the susceptible gene of psoriasis by studying the map the relationship between psoriasis and HLA vulgaris of the Mongolian, for the future of psoriasis related research provide valuable information for the diagnosis, treatment of psoriasis gene level, prognosis evaluation and related research lays the theory. Methods: This study chose unrelated, more than 3 generations of continuous life in the territory of Inner Mongolia Mongolian population, extraction of blood cell genomic DNA by polymerase chain reaction sequence specific primers (PCR-SSP) for patients with Mongolian psoriasis vulgaris and 81 cases of normal Mongolia 100 cases of HLA-Cw and DRB1 alleles were genotyped. According to the frequency distribution of HLA allele between the patients group and control group, using statistical software SPSS13.0 2 test, analysis between the two groups of allele frequency differences. Results: there were 10 HLA-Cw alleles. 12 HLA-DRB1 alleles. These alleles were analyzed statistically according to the difference of the frequency distribution of the psoriasis group and normal group of genes that have significant differences in the allele is: HLA-Cw*04, Cw*06, DRB1*04 and DRB1*07 alleles. Among them, the psoriasis group HLA-Cw*06, DRB1*07 allele frequency was significantly higher than normal group, HLA-Cw*04, DRB1*04 allele frequency was significantly lower than that of normal group (Pc < 0.05). The age of onset of HLA-Cw*06 in less than 40 years of family history of psoriasis and negative patients, the DRB1*07 allele frequency was significantly higher than that of The normal group, while the HLA-Cw*04, DRB1*04 allele frequency was significantly lower than that of normal group (Pc < 0.05). In psoriasis and a positive family history of patients aged 40 years and only the HLA-Cw*06 allele frequency was significantly higher than that in normal group (Pc < 0.05).HLA-Cw*04, decrease of Cw*06 and DRB1*04 gene frequency, DRB1*07 the gene frequency of rise in the no family history of psoriasis patients were statistically significant (Pc < 0.05), and a family history of gene frequency in patients with positive Cw*06 was significantly increased (Pc < 0.05). Conclusion: HLA-Cw*06, DRB1*07 allele may be the susceptibility to psoriasis vulgaris in Mongolian population in Inner Mongolia region of.HLA-Cw*04 gene and the DRB1*04 allele may be the pathogenesis of vulgaris psoriasis in Inner Mongolia area of the Mongolian population protection factor.HLA-DRB1*07 allele may be a susceptible age < 40 years old psoriasis gene, HLA-Cw*04 and DRB1*04 alleles may be protective factors of psoriasis at onset age less than 40 years old. Family history of psoriasis is different from that of family history without psoriasis, and HLA-Cw*06 allele may be the common genetic basis of the two.

【学位授予单位】：内蒙古医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R758.63

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