纳米雄黄对皮肤鳞癌A431细胞株抑制增殖及抑制新生血管作用的实验研究

发布时间：2018-03-01 20:10

本文关键词： 纳米雄黄人皮肤鳞状细胞癌A431细胞肿瘤血管生成细胞增殖　出处：《山东中医药大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：对皮肤肿瘤进行中医文献复习，并通过检测人皮肤鳞癌A431细胞用纳米雄黄处理后细胞增殖抑制及血管生长调控因子表达情况，，探讨纳米雄黄抑制血管生长抗肿瘤的作用机理。方法：查询并复习中医文献资料，对皮肤鳞癌A431细胞进行体外培养，按实验分组加药后观察其形态及生长状况变化，并分别用MTT法、免疫组织化学法和RT-PCR法检测加药后细胞增殖抑制、色素上皮源性因子(PEDF)和VEGFmRNA表达情况，探讨纳米雄黄抑制皮肤鳞癌新生血管生成的作用机理。结果：纳米雄黄可以抑制人皮肤鳞癌A431细胞增殖，且与顺铂联合应用有协同作用，并发现纳米雄黄抑制A431细胞增殖的作用与上调PEDF的表达和下调VEGF的表达以抑制新生血管生成有关。结论：体外实验表明纳米雄黄可显著抑制人皮肤鳞癌A431细胞增殖，并通过抑制其新生血管生成达到抗肿瘤细胞生长的效果，与顺铂有协同作用。
[Abstract]:Objective: to review the literatures of traditional Chinese medicine (TCM) on skin tumors and to detect the inhibition of proliferation and the expression of vascular growth regulation factor (VEGF) in human skin squamous cell carcinoma A431 cells treated with nano-realgar. To explore the mechanism of inhibiting angiogenesis and anti-tumor by nanometer realgar. Methods: the A431 cells of cutaneous squamous cell carcinoma were cultured in vitro. The morphology and growth of A431 cells were observed by MTT. Immunohistochemical method and RT-PCR method were used to detect the expression of PEDFand VEGFmRNA, and to explore the mechanism of inhibiting angiogenesis of skin squamous cell carcinoma by nano-realgar. Results: Nano-realgar could inhibit the proliferation of human skin squamous cell carcinoma A431 cells and had synergistic effect with cisplatin. It was also found that the inhibitory effect of nano-realgar on the proliferation of A431 cells was related to the up-regulation of PEDF expression and down-regulation of VEGF expression to inhibit angiogenesis. Conclusion: nano realgar can significantly inhibit the proliferation of human skin squamous cell carcinoma A431 cells in vitro and can inhibit the growth of human skin squamous cell carcinoma A431 cells by inhibiting its neovascularization. It has synergistic effect with cisplatin.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.5;R285.5

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