皮肤癌相关文献复习及纳米雄黄干预P53、Bcl-2在A431细胞中表达的研究

发布时间：2018-03-06 08:44

本文选题：纳米雄黄　切入点：细胞凋亡　出处：《山东中医药大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：考察纳米雄黄对皮肤鳞癌A431细胞凋亡的作用，探讨纳米雄黄诱导肿瘤细胞凋亡和抑制增殖的机理。方法：本实验主要包括纳米雄黄通过机械研制法制备，A431细胞的形态学观察，运用免疫组化法、RT-PCR技术探讨纳米雄黄诱导人皮肤鳞癌A431细胞凋亡和抑制增殖的作用机理。结果：纳米雄黄可以明显诱导人皮肤鳞癌A431细胞的凋亡，且与顺铂联合应用有协同作用，并发现纳米雄黄诱导肿瘤人皮肤鳞癌A431细胞凋亡的机理与上调P53的表达和下调Bcl-2的表达有关。结论:纳米雄黄可有效诱导皮肤鳞癌A431细胞发生凋亡。纳米雄黄可以通过诱导肿瘤细胞凋亡等途径实现抗肿瘤作用，与顺铂联合用药，可产生协同作用。
[Abstract]:Objective: to investigate the effect of nanometer realgar on apoptosis of skin squamous cell carcinoma A431 cells. To explore the mechanism of inducing apoptosis and inhibiting proliferation of tumor cells by nano-realgar. Methods: this experiment mainly includes the morphological observation of the preparation of A431 cells by mechanical preparation of nano-realgar. The mechanism of nano-realgar induced apoptosis and inhibition of proliferation of human cutaneous squamous cell carcinoma A431 cells was studied by immunohistochemistry and RT-PCR. Results: nano realgar could induce apoptosis of human skin squamous cell carcinoma A431 cell line. And synergistic effect with cisplatin. It was also found that the mechanism of apoptosis induced by nano-realgar was related to the up-regulation of p53 expression and down-regulation of Bcl-2 expression. Conclusion: Nano-realgar can effectively induce apoptosis of human skin squamous cell carcinoma A431 cells. The anti-tumor effect was achieved by inducing apoptosis of tumor cells. In combination with cisplatin, synergistic effects can be produced.
【学位授予单位】：山东中医药大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.5

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