对称性肢端角化病超微结构变化及角蛋白1的表达研究

发布时间：2018-03-18 02:29

本文选题：角蛋白　切入点：对称性肢端角化病　出处：《重庆医学》2017年33期 　论文类型：期刊论文

【摘要】：目的探讨对称性肢端角化病(SAK)皮损超微结构变化及角蛋白(KRT)1的表达。方法选取福建医科大学附属泉州第一医院及东莞市第六人民医院门诊SAK患者13例为研究对象,组织病理标本取材于手腕部位,皮损组织取材前2个月内均未外用维甲酸制剂或皮质类固醇制剂或中药制剂。健康对照皮肤为整形美容手术切除正常皮肤12例。采用透射电镜观察SAK患者皮损超微结构变化,免疫组化法检测13例SAK患者皮损及12例健康者皮肤组织中KRT1的表达。结果 SAK患者皮肤组织超微结构表现为角质化包膜连续性中断,角质层、棘层上部和颗粒层细胞核周围角蛋白丝显著聚集。KRT1在SAK皮损及正常皮肤组织中棘层、颗粒层及角质层均有表达,胞质及胞膜染色多见;KRT1在SAK皮损组织中表达明显高于正常皮肤(t=2.210,P=0.038)。结论 SAK皮损超微结构特点为表皮角蛋白丝分化异常,可能与KRT1过表达有关。
[Abstract]:Objective to investigate the ultrastructural changes of skin lesions and the expression of keratin KRT1 in the skin of patients with symmetrical acrokeratosis. Methods Thirteen SAK patients in Quanzhou first Hospital affiliated to Fujian Medical University and 6th people's Hospital in Dongguan City were selected as subjects. Histopathological specimens were taken from the wrist, No retinoic acid preparation, corticosteroid preparation or Chinese medicine preparation were used outside the skin within 2 months before the skin lesion was taken. The normal skin was resected by plastic surgery in 12 cases of healthy control skin. The ultrastructural changes of skin lesions in SAK patients were observed by transmission electron microscope (TEM). Immunohistochemical method was used to detect the expression of KRT1 in skin lesions of 13 patients with SAK and 12 healthy subjects. Results the ultrastructure of skin tissue of SAK patients was characterized by continuous disruption of keratinized capsule and cuticular layer. Keratin filaments in the upper part of the spinous layer and the granular layer were significantly aggregated. KRT1 was expressed in the spinous layer, granular layer and cuticle in the lesions of SAK and normal skin tissue. Cytoplasmic and cellular membrane staining showed that the expression of KRT1 in SAK lesions was significantly higher than that in normal skin. Conclusion the ultrastructural features of SAK lesions are abnormal differentiation of keratin filaments, which may be related to the overexpression of KRT1.
【作者单位】：福建医科大学附属泉州第一医院皮肤科;广东省东莞市第六人民医院皮肤科;
【基金】：广东省医学科学技术研究基金资助项目(A2016025) 东莞市社会科技发展资助项目(2014108101008) 泉州市科技计划资助项目(2015Z39)
【分类号】：R758.53

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