慢性光化性皮炎的光生物学试验及有关机理的研究

发布时间：2018-03-24 11:32

本文选题：慢性光化性皮炎　切入点：最小红斑量　出处：《昆明医科大学》2012年硕士论文

【摘要】：目的利用云南地区慢性光化性皮炎(chronic actinic dermatitis, CAD)发病率高的优势,对云南地区CAD患者和正常人的最小红斑量和光斑贴试验的差异进行测定和分析,并探讨IL-6、TNF-a与CAD患者发病的相关性。方法采用SUV1000型日光模拟器对21例CAD患者和40例正常对照者进行最小红斑量的测定,并采用SS-03AB型紫外线光疗仪和标准光斑贴试验试剂盒对21例CAD患者和21例正常对照者进行光斑贴试验(光源：UVA5J/cm2)应用ELISA的方法检测CAD患者、正常对照者血清中IL-6、TNF-a的含量。应用SPSS17.0软件对数据资料进行统计分析。结果1、CAD患者组的UVA-MED、UVB-MED分别为26.05J/cm2、28.32mJ/cm2;正常对照组的UVA-MED、UVB-MED分别为50.06J/cm2、38.25mJ/cm2. CAD患者的UVA-MED、UVB-MED较正常人对照组明显降低,P值均0.05。正常对照组内UVA-MED和UVB-MED两性间差异无统计学意义,P均0.05。2、CAD患者、正常人中光斑贴试验阳性率分别为57.1%,47.6%。CAD患者最常见的光变应原为芳香混合物(31.25%)、D-松萝酸(12.50%)和木材合成物(12.50%)；正常人最常见的光变应原为芳香混合物(23.08%)、D-松萝酸(15.38%)、秘鲁香脂(15.38%)。经检验CAD患者与正常人对照组的光斑贴试验结果差异无统计学意义(P0.05)。3、IL-6在CAD患者组血清中为0.953±0.198(pg/ml),低于正常人血清中(3.165±0.772pg/ml)的水平,差异有统计学意义(P0.05)。CAD患者组血清中TNF-a和正常人对照组血清水平无明显差异,无统计学意义(P0.05)。结论1、最小红斑量的测定提示CAD的发病与UVA、UVB的照射有关,其中UVA更为敏感。2、CAD患者及正常人对照组都对多种(光)接触性变应原呈阳性反应。 3、CAD的发病可能和IL-6的降低有一定的关系。
[Abstract]:Objective to measure and analyze the difference of minimal erythema amount and light spot patch test between CAD patients and normal subjects in Yunnan province by using the high incidence rate of chronic actinic dermatitis (CAD), and to explore the correlation between IL-6 actinic TNF-a and the incidence of CAD. Methods the minimal erythema was measured in 21 patients with CAD and 40 normal controls by using SUV1000 solar simulator. Using SS-03AB ultraviolet phototherapy instrument and standard spot patch test kit, 21 CAD patients and 21 normal control subjects were tested by ELISA with the light source of: UVA 5J / cm 2. The contents of IL-6 and TNF-a in serum of normal controls were analyzed by SPSS17.0 software. Results 1 the UVA-MED- UVB-MED was 26.05J / cm ~ (-2) (28.32mJ / cm ~ (-2)) in CAD group and 50.06J / cm ~ (-2) UVB-MED was 58.25mJ / cm ~ (2) in normal control group. The value of UVA-MEDUVB-MED in CAD patients was significantly lower than that in normal control group (0.05). There was no significant difference between UVA-MED and UVB-MED in normal control group (P < 0.05). The most common photoallergens in CAD patients were the aromatic mixture of 31. 25% D- arboxylic acid 12.50) and wood compound 12. 50%, respectively. The most common photoallergens in normal people were the aromatic mixture of 23. 08D-pinealic acid 15.38%, Peruvian balsamic acid 15. 38%. There was no significant difference in the results of spot patch test between CAD patients and normal controls. The serum level of IL-6 in CAD patients was 0.953 卤0.198 mg / ml, which was lower than that in normal controls (3.165 卤0.772 PG / ml). There was no significant difference in serum levels of TNF-a between the patients with P0.05 and the control group, and there was no significant difference in the serum levels between the two groups. Conclusion 1. The determination of minimal erythema quantity indicates that the pathogenesis of CAD is related to the exposure to UVB, and UVA is more sensitive to UVA and normal control group is positive for many (light) contact allergens. 3 the incidence of IL-6 may be related to the decrease of IL-6.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R758.14

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