麻风皮损中Th17细胞及RORγt的表达及意义

发布时间：2018-03-28 05:06

  本文选题：麻风　切入点：Th17　出处：《昆明医科大学》2012年硕士论文

【摘要】：背景：麻风病是由麻风分枝杆菌引起皮肤和外周神经受累的慢性传染病,是导致人类致残的主要疾病之一。人类是麻风分枝菌的主要宿主和传染源,未经治疗的麻风病人(主要是多菌型患者)可以通过呼吸道和皮肤进行传染。但有研究表明,在与传染性麻风患者同等接触的条件下,只有少数人发病,这是因为麻风患者存在着不同程度的细胞免疫缺陷,但其细胞免疫缺陷的确切机制尚不明了。近来Th17细胞逐渐被认为是一个独立的辅助T细胞亚群,具有抑制其他T细胞活化的功能,即具有免疫调节或具有抑制作用。孤独核受体(Orphan nuclear receptor,RORyt)是控制Th17细胞分化的转录因子。有研究报道,结核病的发病与Th17细胞及RORyt有关。麻风病与结核病有许多相似之处。因此,本文主要研究Thl7细胞及RORyt与麻风病发病的关系。目的：研究麻风患者皮损中Th17细胞及RORyt的表达,阐明Th17细胞及RORyt与麻风发病的关系。 方法：1、麻风患者组：选择30例麻风患者,根据国际标准将患者分为TT、BT、 BB、BL及LL型,用免疫组化测皮损中Th17细胞及RORγt的分布。 2、健康对照组：为门诊健康体检者20例,用免疫组化测皮损中Th17细胞及RORyt的分布。 结果：1、Th17细胞主要位于麻风皮损真皮层,并表达于胞浆；正常对照皮肤中Th17细胞数量减少或无。BT型和TT型中Thl7细胞分布在肉芽肿及其周围。LL型和BL型Th17细胞分布在侵润的巨噬细胞间。本实验结果显示,Th17细胞分布与巨噬细胞有关,提示Th17细胞和组织细胞之间可能存在相互作用。 2、RORyt主要位于麻风皮损的真皮层,且表达于胞浆；正常对照皮肤中RORyt数量减少或无。BT型和TT型中RORyt分布在肉芽肿及其周围。LL型和BL型中RORyt分布在侵润的巨噬细胞间。 结论：1、Th17细胞是麻风病的重要参与者2、提示Th17细胞与组织细胞之间存在相互作用。3、Th17细胞在LL、BL、BT、TT中呈逐渐增高的趋势,提示Th17细胞可能对麻风患者有保护作用。4、实验组RORyt高于健康对照组,提示麻风患者发病可能与RORyt相关,并且通过调节Th17细胞的分化而发生作用。RORyt表达在LL、BL、BT、TT中呈逐渐增高的趋势,提示RORyt可能对麻风患者有保护作用。5、Th17细胞具有抵抗细胞外感染的作用,能在局部引起炎症,抵抗外来病原体。
[Abstract]:Background: leprosy is a chronic infectious disease of skin and peripheral nerve caused by Mycobacterium leprosy. It is one of the major diseases causing human disability. Human is the main host and source of infection of Mycobacterium leprosy. Untreated leprosy patients (mainly multicellular) can be transmitted through the respiratory tract and skin. But studies have shown that only a small number of people develop disease under the same conditions as people with infectious leprosy. This is due to varying degrees of cellular immune deficiency in leprosy patients, but the exact mechanism of cellular immune deficiency remains unclear. Recently, Th17 cells have been recognized as an independent helper T cell subgroup. It has the function of inhibiting the activation of other T cells, that is, it has immunomodulation or inhibitory effect. Orphan nuclear receptor RORytis a transcription factor controlling the differentiation of Th17 cells. The pathogenesis of tuberculosis is related to Th17 cells and RORyt. There are many similarities between leprosy and leprosy. Therefore, the relationship between Thl7 cells and RORyt and leprosy was studied. Objective: to study the expression of Th17 cells and RORyt in leprosy skin lesions. To elucidate the relationship between Th17 cells and RORyt and leprosy. Methods Twenty patients with leprosy were divided into BTT, BBBBL and LL according to international standards. The distribution of Th17 cells and ROR 纬 t in lesions were detected by immunohistochemistry. 2. The distribution of Th17 cells and RORyt in skin lesions were detected by immunohistochemistry. Results the Th17 cells were mainly located in the dermis of leprosy and expressed in the cytoplasm. The number of Th17 cells in normal control skin decreased or did not. BT type and TT type Thl7 cells distributed in granuloma and its surrounding. LL and BL types of Th17 cells distributed in infiltrated macrophages. The results showed that the distribution of Th 17 cells was related to macrophages. These results suggest that there may be interaction between Th17 cells and histocytes. 2RORyt was mainly located in the dermis of leprosy and expressed in cytoplasm, and RORyt in normal skin decreased or did not exist in BT type and TT type in granuloma and its surrounding. LL and BL types, RORyt was distributed between invading macrophages. Conclusion Th17 cells are important participants in leprosy, suggesting that the interaction between Th17 cells and histiocytes. The results suggest that Th17 cells may have protective effect on leprosy patients. The RORyt of experimental group is higher than that of healthy control group, suggesting that the pathogenesis of leprosy patients may be related to RORyt, and the expression of .RORyt may increase gradually by regulating the differentiation of Th17 cells. The results suggest that RORyt may have protective effect on leprosy patients. 5. Th17 cells can resist extracellular infection, and can cause inflammation locally and resist foreign pathogens.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R755.01

【引证文献】

相关硕士学位论文 前1条

1 吴成;Th1、Th2和Th17相关细胞因子在麻风病中的水平和意义[D];昆明医科大学;2013年

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本文编号：1674868