皮肤鳞状细胞癌组织中miR-21、miR-31、Ras GTP酶激活蛋白1表达与人乳头瘤病毒的相关性研究

发布时间：2018-03-31 15:40

本文选题：人乳头瘤病毒　切入点：皮肤鳞状细胞癌　出处：《临床皮肤科杂志》2017年08期

【摘要】：目的:测定皮肤鳞状细胞癌(CSCC)组织中人乳头瘤病毒(HPV)、miR-21及miR-31的表达量,分析HPV感染与miRNA之间的相关性,并探讨miR-31可能的致癌机制。方法:采用HPV DNA检测试剂盒和巢式聚合酶链式反应(PCR)检测CSCC组和正常对照组中HPV表达情况,实时荧光定量PCR分别测定HPV阳性和阴性CSCC组织中miR-21及miR-31的表达量,免疫组化检测CSCC组织中Ras GTP酶激活蛋白(RASA)1表达水平。结果:CSCC组织中HPV检出率为18.29%(15/82)。miR-21和miR-31在CSCC组织中相对表达量分别为0.2750±0.2280和0.0130±0.0220,均高于正常对照组(0.0110±0.0190、0.0002±0.0002),差异均有统计学意义(P均0.05);RASA1蛋白在CSCC组织中表达明显减少(P0.05)。在HPV阴性组织中RASA1蛋白表达与miR-31水平呈负相关(r=-0.588,P0.05)。结论:HPV可能在CSCC发展早期起一定作用;miR-31可能是HPV相关性miRNA;高表达的miR-31可能通过下调RASA1促进细胞增殖,参与CSCC的发生及发展。
[Abstract]:Objective: to detect the expression of human papillomavirus (HPVV) miR-21 and human papillomavirus (miR-31) in skin squamous cell carcinoma (CSCC) tissues, and to analyze the correlation between HPV infection and miRNA. Methods: HPV DNA assay kit and nested polymerase chain reaction (PCR) were used to detect the expression of HPV in CSCC group and normal control group. Real-time fluorescence quantitative PCR was used to detect the expression of miR-21 and miR-31 in HPV positive and negative CSCC tissues. Immunohistochemistry was used to detect the expression of Ras GTP enzyme activator protein (RASA) 1 in CSCC. Results the relative expression levels of 18.29%(15/82).miR-21 and miR-31 in CSCC were 0.2750 卤0.2280 and 0.0130 卤0.0220, respectively, which were significantly higher than those in normal control group (0.0110 卤0.0190,0.0002 卤0.0002). In HPV negative tissues, the expression of RASA1 protein was negatively correlated with the level of miR-31. Conclusion CSCC may play a role in HPV related miRNAs in early stage of CSCC development, and high expression of miR-31 may play a role in the development of CSCC. Can promote cell proliferation by down-regulating RASA1, Participate in the occurrence and development of CSCC.
【作者单位】：南京明基医院皮肤科;东南大学附属中大医院皮肤科;
【基金】：教育部留学回国人员科研启动基金(20121707)资助项目
【分类号】：R739.5

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