蒺藜防白丸对体外培养黑素细胞生物学特性的影响

发布时间：2018-04-05 10:32

本文选题：蒺藜防白丸　切入点：黑素细胞　出处：《中国皮肤性病学杂志》2016年09期

【摘要】：目的研究蒺藜防白丸对黑素细胞(melanocytes,MC)的形态、增殖、酪氨酸酶活性及黑素合成的影响。方法采用倒置显微镜观察细胞形态,四甲基偶氮唑蓝(MTT)法测定蒺藜防白丸对黑素细胞增殖的影响,多巴氧化法测定酪氨酸酶活性,NaOH法测定黑素含量。结果随着蒺藜防白丸浓度的升高,黑素细胞树突变粗短,且数量减少;低浓度(0.001~0.5mg/mL)的蒺藜防白丸对黑素细胞增殖影响不显著(P0.05),高浓度时(1mg/mL和5mg/mL)抑制作用明显(P0.05);低浓度的蒺藜防白丸可以提高酪氨酸酶活性,其中0.05mg/mL时酪氨酸酶活性最高(P0.05),然而当蒺藜防白丸浓度继续增大,则会对酪氨酸酶活性产生抑制作用;蒺藜防白丸对黑素细胞黑素合成也有类似的影响。结论蒺藜防白丸高浓度时抑制黑素细胞的增殖、酪氨酸酶活性及黑素合成;低浓度时促进酪氨酸酶活性及黑素合成。
[Abstract]:Objective to study the anti white Tribulus terrestris Pill on melanocytes (melanocytes, MC) the effect of morphology, proliferation, tyrosinase activity and melanin synthesis method. Cell morphology was observed by inverted microscope, four methyl thiazolyl tetrazolium (MTT) method for the determination of anti white tribulus pill effect on melanocyte proliferation, tyrosinase activity was measured by DOPA oxidation method, NaOH method for the determination of melanin content. Results with increasing concentrations of anti white Tribulus terrestris pill, melanocytes mutation tree thick and short, and reduce the number of low concentration; (0.001~0.5mg/mL) the influence of anti white Tribulus terrestris Pill on melanocyte proliferation was not significant (P0.05), high concentration (1mg/mL and 5mg/mL) significantly inhibited (P0.05); low concentration of anti white tribulus pills can increase the activity of tyrosinase, tyrosinase activity and the 0.05mg/mL (P0.05), while the highest concentration of anti white tribulus pill continues to increase, it will cause the inhibition of tyrosinase activity; anti white tribulus Conclusion: pill has a similar effect on melanin synthesis. Conclusion Tribulus terrestris white pill can inhibit the proliferation of melanocytes, tyrosinase activity and melanin synthesis at high concentration, and promote tyrosinase activity and melanin synthesis at low concentration.

【作者单位】：郑州大学附属郑州中心医院;
【分类号】：R758.41

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