三氯乙烯药疹样皮炎患者血清微量元素含量及肝代谢酶基因表达水平的测定

发布时间：2018-04-05 08:23

本文选题：三氯乙烯　切入点：药疹样皮炎　出处：《中国皮肤性病学杂志》2014年12期

【摘要】：目的探讨三氯乙烯(TCE)药疹样皮炎患者血中微量元素和肝代谢酶基因表达水平改变。方法采用全自动生化分析仪测定病例组和对照组血清中Ca,Mg,Fe和P等微量元素含量。采用实时荧光定量PCR(real-time quantitative PCR)技术检测外周血中CYP1A2,CYP2E1,CYP3A4和CYP2C9 mRNA表达水平。结果 TCE皮炎患者血清Ca水平下降明显,治疗后有所提高,差异有统计学意义(P0.05);血清Fe含量病例组治疗后较治疗前下降明显,差异有统计学意义(P0.05);血清P水平病例组治疗前与对照组比较显著下降,治疗后恢复正常,差异有统计学意义(P0.05);血清Mg含量变化不大,稍有下降,组间差异无统计学意义(P0.05)。TCE皮炎患者外周血肝代谢酶CYP1A2,CYP2E1,CYP3A4和CYP2C9 mRNA表达水平均提高,与对照组比较差异有统计学意义(P0.05)。结论三氯乙烯可导致体内微量元素含量变动和肝代谢酶基因表达水平改变,提示某些微量元素和肝代谢酶基因可能参与TCE的毒性代谢机制。
[Abstract]:Objective to investigate the changes of serum trace elements and gene expression of liver metabolic enzymes in patients with drug rash dermatitis by TCE-trichloroethylene.Methods the contents of trace elements CaMg-Fe and P in serum of patients and control group were determined by automatic biochemical analyzer.The expression of CYP2E3A4 and CYP2C9 mRNA in peripheral blood was detected by real-time fluorescence quantitative PCR(real-time quantitative.Results the level of serum Ca in patients with TCE dermatitis decreased significantly after treatment, and the difference was statistically significant (P 0.05).The difference was statistically significant (P 0.05), the serum P level decreased significantly before treatment and returned to normal after treatment, and the difference was statistically significant (P 0.05).There was no significant difference in the expression of CYP1A2CY CYP2E3A4 and CYP2C9 mRNA in peripheral blood of patients with TCE dermatitis.Conclusion trichloroethylene can cause the change of trace element content and the expression level of liver metabolic enzyme gene in vivo, suggesting that some trace elements and liver metabolic enzyme gene may be involved in the toxic metabolic mechanism of TCE.
【作者单位】：深圳市龙岗区人民医院皮肤科;深圳市疾病预防控制中心;深圳市龙岗区人民医院检验科;
【分类号】：R758.21

【参考文献】