黄芩苷联合NB-UVB对黑素细胞黑素合成及酪氨酸酶活性的影响

发布时间：2018-04-08 23:19

本文选题：黑素细胞　切入点：黄芩苷　出处：《吉林大学学报(医学版)》2014年05期

【摘要】：目的:观察体外培养的人正常黑素细胞经黄芩苷和窄波紫外线(NB-UVB)处理后细胞黑素合成及酪氨酸酶活性的变化,为白癜风的新疗法研究提供理论依据。方法:人正常黑素细胞分为空白对照组、不同浓度黄芩苷组、不同剂量NB-UVB组及黄芩苷和NB-UVB联合组。应用MTT法检测各组黑素细胞增殖率,应用NaOH裂解法测定黑素水平,应用多巴氧化法测定酪氨酸酶活性。结果:与空白对照组比较,1×10-4 mol·L-1黄芩苷及30mJ·cm-2 NB-UVB组黑素细胞增殖率差异无统计学意义(P0.05)。与空白对照组比较,黄芩苷组、NB-UVB组及联合组黑素水平及酪氨酸酶活性均增加(P0.05或P0.01);与黄芩苷组和NB-UVB组比较,联合组黑素水平和酪氨酸酶活性均增加(P0.01)。结论:黄芩苷与NB-UVB联合应用较2种方法单独应用具有更好的促黑素合成效果。
[Abstract]:Aim: to observe the changes of melanin synthesis and tyrosinase activity in cultured human normal melanocytes treated with baicalin and NB-UVB in vitro, and to provide a theoretical basis for the study of new therapy for vitiligo.Methods: human normal melanocytes were divided into blank control group, different concentration baicalin group, different dosage NB-UVB group and baicalin and NB-UVB combined group.The proliferation rate of melanocytes in each group was detected by MTT assay, melanin level was measured by NaOH lysis method, tyrosinase activity was measured by dopa oxidation method.Results: there was no significant difference in proliferation rate of melanocytes between 1 脳 10 ~ (-4) mol ~ (-1) baicalin group and 30mJ cm-2 NB-UVB group compared with control group (P 0.05).Compared with the blank control group, the melanin level and tyrosinase activity in baicalin group and combination group increased P 0.05 or P 0.01, respectively, and compared with baicalin group and NB-UVB group, the melanin level and tyrosinase activity in combination group increased P 0.01.Conclusion: baicalin combined with NB-UVB has better effect of melanin synthesis than that of two methods alone.
【作者单位】：吉林大学第一医院皮肤科;
【基金】：吉林省科技厅科技发展计划项目资助课题(20110478) 吉林省中医药管理局中医药科技项目资助课题(2006)
【分类号】：R758.41

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