红景天苷对人皮肤黑色素瘤细胞A375侵袭能力影响的实验研究

发布时间：2018-04-21 22:31

本文选题：红景天苷 + 人皮肤黑色素瘤细胞A375　；参考：《青海大学》2012年硕士论文

【摘要】：目的黑色素瘤是目前最具侵润和迁移能力的恶性实体瘤之一，该肿瘤扩散迅速，患者可在诊断数月后死亡。黑色素瘤对放化疗均不敏感，目前手术依然是本病的重要的治疗手段，，目前最新的基因治疗为黑色素瘤的治疗提供了新的方向，成为当今世界颇有前途的研究领域。目前我省对红景天的药理作用开发主要集中在抗疲劳、抗缺氧的研究，利用红景天进行抗肿瘤领域的基础研究目前尚无报道，本研究采用青海地区特产唐古拉红景天提取物作用于人黑素瘤细胞株A375，探讨红景天苷对人皮肤黑色素瘤细胞株A375侵袭能力的影响。方法采用细胞培养技术培养的人皮肤黑色素瘤细胞A375，经不同浓度（0.01、0.10、1.00、10.00、100.00μg/mL）红景天苷处理后，应用细胞黏附实验检测人皮肤黑色素瘤细胞A375对基膜黏附的影响；应用Transwell小室模型，观察人皮肤黑色素瘤细胞A375在红景天苷影响下侵袭、迁移能力的改变。并与对照组（红景天苷未处理组）进行比较和分析。实验结果不同浓度的红景天苷处理的A375细胞的黏附、侵袭和迁移能力均被抑制，差异具有统计学意义（P0.05），并且随红景天苷浓度的增高其抑制作用增强，不同浓度组间差异显著，有统计学意义（P0.05）。结论红景天苷能够抑制A375细胞黏附、迁移和侵袭
[Abstract]:Objective melanoma is one of the most invading and migrating malignant solid tumors. Melanoma is not sensitive to radiotherapy and chemotherapy. At present, surgery is still an important method for the treatment of melanoma. The latest gene therapy has provided a new direction for the treatment of melanoma and has become a promising research field in the world. At present, the pharmacological development of Rhodiola in our province is mainly focused on anti-fatigue and anti-hypoxia studies, and there is no report on the basic research in the field of anti-tumor by using Rhodiola. The aim of this study was to investigate the effect of salidroside on the invasion of human skin melanoma cell line A375. Methods Human skin melanoma cells A375 were cultured by cell culture technique and treated with salidroside at different concentrations of 0.01g / mL. The effect of human skin melanoma cell line A375 on basement membrane adhesion was detected by cell adhesion assay. Transwell chamber model was used to study the effect of A375 on the adhesion of human skin melanoma cells to the basement membrane, and to observe the effect of different concentrations of salidroside on the adhesion of human skin melanoma cells to the basement membrane. To observe the change of invasion and migration ability of human skin melanoma cell A375 under the influence of salidroside. And compared with the control group (salidroside untreated group). Results the adhesion, invasion and migration of A375 cells treated with different concentrations of salidroside were inhibited, and the difference was statistically significant (P 0.05). There was significant difference in P0.05. Conclusion salidroside can inhibit the adhesion, migration and invasion of A375 cells.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.5

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