特应性皮炎患儿外周血巨噬细胞游走抑制因子及IL-17和IL-23的表达及其相关性研究

发布时间：2018-04-23 22:03

本文选题：皮炎 + 特应性　；参考：《中国全科医学》2014年08期

【摘要】：目的检测特应性皮炎(AD)患儿外周血单个核细胞(PBMCs)中巨噬细胞游走抑制因子(MIF)、IL-17和IL-23 mRNA表达水平及血清水平,并探讨其相关性。方法选择2010年4—11月在我院门诊就诊的36例AD患儿为病例组,同期在我院体检正常儿33例为对照组,采用实时荧光定量反转录聚合酶链反应(RT-PCR)检测两组受试者PBMCs中MIF、IL-17和IL-23 mRNA表达水平,酶联免疫吸附试验检测其血清MIF、IL-17和IL-23水平。结果病例组PBMCs中MIF、IL-17和IL-23 mRNA表达水平分别为(5.93±2.35)、(6.76±1.87)和(3.21±0.73),对照组分别为(1.83±0.69)、(1.77±0.51)和(0.92±0.22),病例组PBMCs中MIF、IL-17和IL-23mRNA表达水平均高于对照组(P0.01)。病例组血清MIF、IL-17和IL-23水平分别为(34.92±8.73)μg/L、(33.29±5.70)ng/L和(54.62±9.69)ng/L,对照组分别为(9.80±2.21)μg/L、(11.71±2.08)ng/L和(18.97±4.39)ng/L,病例组血清MIF、IL-17和IL-23水平均高于对照组(P0.01)。线性相关分析结果显示,AD患儿PBMCs中MIF mRNA表达水平(r=0.418,P=0.011)及血清MIF水平(r=0.419,P=0.011)与疾病严重程度(SCORAD)评分均呈正相关;PBMCs中MIF mRNA表达水平与IL-17 mRNA、IL-23 mRNA表达水平均呈正相关(rIL-17 mRNA=0.395,P=0.017;rIL-23 mRNA=0.422,P=0.010);血清MIF水平与IL-17、IL-23水平均呈正相关(rIL-17=0.407,P=0.014;rIL-23=0.375,P=0.024)。结论 MIF在AD患儿中高表达,且与IL-17、IL-23密切相关,炎性因子间的相互作用可能共同参与了AD的发生、发展。
[Abstract]:Objective to detect the expression of IL-17 and IL-23 mRNA in peripheral blood mononuclear cells (PBMCs) of children with atopic dermatitis (AD) and to explore the correlation between IL-17 and IL-23 mRNA. Methods 36 patients with AD were selected as the case group and 33 normal children as the control group. The expression levels of MIF-1 IL-17 and IL-23 mRNA in PBMCs were detected by real-time fluorescence quantitative reverse transcriptase polymerase chain reaction (RT-PCR), and the serum levels of MIF-1 IL-17 and IL-23 were detected by enzyme-linked immunosorbent assay (Elisa). Results the expression levels of MIFIL-17 and IL-23 mRNA in PBMCs were 5.93 卤2.35, 6.76 卤1.87) and 3.21 卤0.73, respectively, and those in control group were 1.83 卤0.69, 1.77 卤0.51) and 0.92 卤0.22, respectively. The expression levels of MIFIL-17 and IL-23mRNA in PBMCs in the case group were higher than those in the control group (P 0.01). The serum levels of IL-17 and IL-23 were 34.92 卤8.73 渭 g / L, 33.29 卤5.70)ng/L and 54.62 卤9.69 ng / L in the case group, and 11.71 卤2.08)ng/L and 18.97 卤4.39 ng / L in the control group, respectively. The levels of IL-17 and IL-23 in the case group were higher than those in the control group (P 0.01). The results of linear correlation analysis showed that the expression level of MIF mRNA in PBMCs and the level of serum MIF were positively correlated with the severity of disease and the level of MIF mRNA and IL-17 mRNA-IL-23 mRNA expression. Both the levels of MIF mRNA and IL-17 mRNA-23 mRNA were positively correlated with the serum level of rIL-17 mRNA0.395mRNA-0.017rIL-23 mRNA-0.422P0.010; There was a positive correlation between the level of MIF and the level of IL-23. There was a positive correlation between the level of rIL-17 and the level of IL-23. Conclusion the high expression of MIF in AD children is closely related to IL-17 and IL-23. The interaction between inflammatory factors may be involved in the occurrence and development of AD.
【作者单位】：滨州医学院附属医院皮肤科;滨州医学院附属医院检验科;滨州医学院临床学院内科学教研室;中国医科大学附属第一医院皮肤科;沈阳市第七人民医院皮肤科;
【基金】：山东省科技发展计划(2011YD18069) 山东省自然科学基金资助项目(ZR2010HQ013) 山东省高等学校科技计划(J10LF90) 山东省医药卫生科技发展计划(2011QZ003) 滨州市科技发展计划(2011ZC0914)
【分类号】：R758.2

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