利用Biolog微生物自动分析系统建立常见皮肤癣菌的鉴定数据库

发布时间：2018-04-24 06:21

本文选题：皮肤癣菌 + 鉴定　；参考：《复旦大学》2010年硕士论文

【摘要】： 目的利用Biolog微生物自动分析系统建立常见皮肤癣菌的鉴定数据库,探讨该系统鉴定皮肤癣菌的应用前景。方法采用表型及DNA测序的方法,将临床收集的菌株鉴定至种；选取红色毛癣菌、须癣毛癣菌、断发毛癣菌、犬小孢子菌、石膏样小孢子菌和絮状表皮癣菌6种常见皮肤癣菌接种于FF微量板,记录皮肤癣菌对95种不同碳源的利用情况,描述其各自的生长反应谱,建立鉴定数据库。结果 6种皮肤癣菌对一些碳源的利用具有明显的差别,通过是否利用棉子糖可以将须癣毛癣菌、断发毛癣菌同其它4种皮肤癣菌进行区分；而葵二酸可以区分须癣毛癣菌、断发毛癣菌；通过延胡索酸和琥珀酸可以将红色毛癣菌同石膏样小孢子菌、絮状表皮癣菌和犬小孢子菌进行区分；通过是否利用丙氨酸和苯丙氨酸可以对石膏样小孢子菌进行鉴定。而糊精的利用可以区分絮状表皮癣菌和犬小孢子菌。结论 Biolog微生物鉴定系统采用一种特殊的表型鉴定方法,可以对常见皮肤癣菌进行鉴定,初步推测该系统在皮肤癣菌鉴定中的应用前景。
[Abstract]:Purpose The identification database of common dermatophytes was established by Biolog microorganism automatic analysis system, and the application prospect of this system was discussed. Method The clinical collected strains were identified to species by phenotype and DNA sequencing, and Trichophyton rubrum, Trichophyton rubrum, Trichophyton punctatus, Microspora canis were selected. Six common dermatophytes were inoculated on FF microplate to record the utilization of 95 different carbon sources, to describe their growth response spectra, and to establish the identification database. Result There are significant differences in the utilization of some carbon sources among the 6 species of dermatophytes, which can be distinguished from the other four species of Trichophyton dermatophytes by the use of gossypids, while the sundiacid can distinguish the Trichophyton trichophyton from other four species of Trichophyton dermatophytes. Through fumaric acid and succinic acid, Trichophyton rubrum can be distinguished from microsporum gypsum, Trichophyton flocculata and microsporum canis. Whether alanine and phenylalanine can be used to identify microsporum gypsum. The use of dextrin can distinguish between Trichophyton flocculata and microsporum canis. Conclusion Biolog microbial identification system used a special phenotypic identification method to identify common dermatophytes and preliminarily speculated the application prospect of this system in dermatophytes identification.
【学位授予单位】：复旦大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R756

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