组胺对皮肤鳞状细胞癌细胞生长和迁移的影响

发布时间：2018-04-28 06:58

本文选题：组胺 + A细胞株　；参考：《临床皮肤科杂志》2014年05期

【摘要】：目的:探讨组胺处理体外培养的皮肤鳞状细胞癌细胞(A431)对其生物学行为的影响。方法:用细胞活力(CCK-8)法检测不同浓度组胺处理A431细胞的活性,筛选用于本研究的有效组胺浓度。流式细胞仪检测癌细胞增殖周期细胞百分比与细胞增殖指数;划痕、Transwell小室和黏附实验分别检测癌细胞的迁移、侵袭和黏附潜能。结果:与未处理的对照组相比,组胺处理A431细胞在48 h出现显著抑制作用,呈浓度和时间依赖关系。组胺10~4 mol/L组在48 h和72 h处理癌细胞活力比其他浓度组更显著(均P0.01)。组胺10~4mol/L组处理后细胞在G1期比率显著增加,在S期比率明显降低(均P0.05),并抑制A431细胞的迁移、侵袭和黏附(均P0.05)。结论:组胺能有效抑制A431细胞增殖、迁移、侵袭和黏附的生物学行为。
[Abstract]:Aim: to investigate the effect of histamine treatment on the biological behavior of skin squamous cell carcinoma cell line A431. Methods: the activity of A431 cells treated with different concentrations of histamine was determined by CCK-8 assay, and the effective histamine concentration was screened. The cell percentage and cell proliferation index of cancer cells were detected by flow cytometry, and the migration, invasion and adhesion potential of cancer cells were detected by scratch well chamber and adhesion assay, respectively. Results: compared with the untreated control group, histamine treated A431 cells showed a significant inhibitory effect at 48 h, in a dose-dependent and time-dependent manner. The activity of cancer cells treated with histamine 10 ~ 4 mol/L at 48 h and 72 h was significantly higher than that of other concentration groups (all P 0.01). After treatment with histamine 10~4mol/L, the ratio of cells in G1 phase was significantly increased, and the ratio in S phase was significantly decreased (all P 0.05), and the migration, invasion and adhesion of A431 cells were inhibited (all P 0.05). Conclusion: histamine can effectively inhibit the biological behavior of proliferation, migration, invasion and adhesion of A 431 cells.
【作者单位】：天津市第五中心医院手术室;南通大学附属医院皮肤科;南通大学医学院病理学系;
【分类号】：R739.5

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