雷帕霉素对皮肤鳞癌细胞体外转移潜能的影响

发布时间：2018-05-01 09:21

本文选题：雷帕霉素 + 上皮鳞状细胞癌　；参考：《中国药理学通报》2014年04期

【摘要】：目的研究雷帕霉素(rapamycin,RAPA)在体外对上皮鳞癌细胞系A431的增殖、迁移以及侵袭能力的影响。方法体外培养人上皮鳞癌细胞A431,通过噻唑蓝(MTT)实验检测不同浓度的RAPA(5、10、20 nmol·L-1)对A431增殖的影响,并筛选出最适浓度。依此浓度分别通过划痕、Transwell试验检测A431的迁移和侵袭能力变化。经RAPA处理后,采用逆转录-聚合酶链反应(RT-PCR)和Western blot检测A431中骨桥蛋白(osteopontin,OPN)的mRNA和蛋白的表达水平的变化。结果 MTT实验结果显示不同浓度RAPA对A431增殖有抑制作用,且表现浓度依赖性。RAPA作用于A431细胞48 h后,划痕实验及Transwell实验显示A431的迁移和侵袭能力受到明显抑制。RT-PCR和Western blot结果都显示RAPA处理的A431细胞中OPN的表达下调。结论 RAPA可降低鳞癌细胞A431的增殖、迁移以及侵袭能力,其机制可能与RAPA抑制OPN的表达有关。
[Abstract]:Objective to study the effects of rapamycin A on the proliferation, migration and invasion of epithelial squamous cell carcinoma cell line A 431 in vitro. Methods Human epithelial squamous carcinoma cell line A431 was cultured in vitro. The effects of different concentrations of RAPA5 10 ~ (10) nmol L ~ (-1) on the proliferation of human epithelial squamous carcinoma cell line A431 were detected by MTT assay, and the optimum concentration was screened out. The migration and invasiveness of A 431 were detected by scratch Transwell test. After treated with RAPA, the expression of mRNA and protein of osteopontin in A431 was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Results the results of MTT assay showed that the proliferation of A431 cells was inhibited by different concentrations of RAPA, and the proliferation of A431 cells was inhibited by RAPA in a dose-dependent manner. Scratch test and Transwell assay showed that the migration and invasion ability of A431 was significantly inhibited. The results of RT-PCR and Western blot showed that the expression of OPN was down-regulated in A431 cells treated with RAPA. Conclusion RAPA can reduce the proliferation, migration and invasion of squamous cell carcinoma A431, and its mechanism may be related to the inhibition of OPN expression by RAPA.
【作者单位】：安徽医科大学生物化学与分子生物学教研室;
【基金】：国家自然科学基金资助项目(No 81172591)
【分类号】：R739.5

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