不良生活习性对青少年痤疮患者Th17、Treg、sIL-2R及TNF-α表达的调控作用研究

发布时间：2018-05-05 14:19

  本文选题：不良习性 + 免疫失衡　； 参考：《青岛大学》2017年硕士论文

【摘要】：目的本研究选取吸烟、饮酒、睡眠不足三类不良生活习性的青少年为研究对象,重点探讨青少年不良习性与Th17、Treg细胞及相关细胞因子TNF-α、sIL-2R引起的免疫紊乱以及痤疮患病的相关性,对青少年的健康成长具有重要指导价值。方法1、采用现况调查研究方法,以随机整群抽样方法抽取菏泽家政职业学院384名学生作为样本,自拟问卷调查表,进行匿名问卷调查。分析在校青少年不良习性和痤疮患病现状,采用卡方检验和Logistic回归分析探讨青少年不良习性中可能影响痤疮患病的危险因素。2、从2015年5月到2015年9月菏泽家政职业学院参加健康体检的学生中选择60人作为研究对象,共分为三组,吸烟组20人,睡眠不足组20人,对照组20人。用酶联免疫分析(ELISA)方法测定血清中sIL-2R、TNF-α浓度;用流式细胞术测定外周血Th17、Treg细胞比例;用RT-qPCR测定外周血TNF-α、Foxp3、RORγt mRNA水平。采用单因素方差分析和spearson相关分析法探讨吸烟、睡眠不足同sIL-2R、TNF-α、Th17、Treg 之间的关系。3、从2015年10月到2016年8月在单县中心医院皮肤科就诊的青少年痤疮患者中,按照临床诊断情况及Pillsbury分级法选择60名患者,将其分别分为痤疮Ⅰ级组、痤疮Ⅱ级组、痤疮Ⅲ级组、痤疮Ⅳ级组,每组15人,并选取15名健康体检者作为对照组。用酶联免疫分析(ELISA)方法测定血清中sIL-2R、TNF-α浓度;用流式细胞术测定外周血Th17、Treg细胞比例。采用单因素方差分析和spearson相关分析法探讨痤疮严重程度同sIL-2R、TNF-α、Th17、Treg之间的关系。结果1、单因素分析,吸烟、睡眠不足、饮酒都是痤疮的可疑影响因素,二元Logistic回归分析,只有吸烟、睡眠不足是痤疮的危险影响因素。2、吸烟组、睡眠不足组同对照组相比,sIL-2R、TNF-α浓度显著升高,差异有统计学意义;Th17细胞比例、Th17/Treg显著升高,差异有统计学意义;TNF-α、RORγt mRNA相对表达水平显著升高,差异有统计学意义。吸烟量、睡眠不足时间同 sIL-2R、TNF-α、Th17、Th17/Treg 明显正相关。3、各痤疮患者组与对照组相比,sIL-2R、TNF-α浓度显著升高,差异有统计学意义;Th17、Th17/Treg显著升高,差异有统计学意义。痤疮严重程度同sIL-2R、TNF-α、Th17、Th17/Treg 明显正相关。结论本研究从基因、分子水平证明青少年吸烟、饮酒、睡眠不足的不良习性可导致外周血sIL-2R、TNF-α、Th17增高,Th17/Treg比例失衡,外周血RORyt、TNF-αmRNA高表达。在痤疮炎症患者中这些免疫紊乱更为显著。由此表明吸烟、睡眠不足引发的免疫失衡是导致痤疮炎症发生的主要病因。故本课题对青年学子良好生活习性的培养具有重要引导价值。
[Abstract]:Objective to investigate the relationship between unhealthy habits of adolescents, such as smoking, drinking and lack of sleep, and the immune disorder induced by Th17 Treg cells and the related cytokines TNF- 伪 -nsIL-2R, as well as the prevalence of acne, in order to explore the relationship between the unhealthy habits of adolescents and the immune disorder induced by Th17 Treg cells and the related cytokines TNF- 伪 -nsIL-2R. It has important guiding value for the healthy growth of teenagers. Methods 1. 384 students of Heze Home Economics and Vocational College were sampled by random cluster sampling method. To analyze the prevalence of bad habits and acne among adolescents in school. Chi-square test and Logistic regression analysis were used to explore the risk factors of acne prevalence in adolescents. From May 2015 to September 2015, 60 students from Heze Home Economics Vocational College were selected as subjects. They were divided into three groups: smoking group (n = 20), sleep deprivation group (n = 20) and control group (n = 20). The concentration of sIL-2RnTNF- 伪 in serum was determined by Elisa, the percentage of Th17Treg cells in peripheral blood was measured by flow cytometry, and the level of TNF- 伪 Foxp3ROR 纬 t mRNA in peripheral blood was determined by RT-qPCR. Univariate analysis of variance (ANOVA) and spearson correlation analysis were used to investigate the relationship between smoking, sleep deprivation and sIL-2RN- 伪 TNF- 伪 TNF- 伪 Th17 Treg. From October 2015 to August 2016, adolescents with acne were admitted to the Department of Dermatology in Shan County Central Hospital. According to the clinical diagnosis and Pillsbury classification, 60 patients were divided into acne grade 鈪，

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