血管瘤组织中β肾上腺素能受体的密度及亲和力

发布时间：2018-05-06 00:03

本文选题：血管瘤 + 受体　；参考：《中国皮肤性病学杂志》2014年03期

【摘要】：目的研究血管瘤组织中β肾上腺素能受体(β-AR)的密度和亲和力,并探讨其在血管瘤发病中的意义。方法采用放射标记的非选择性β-AR阻滞剂125I-吲哚洛尔、选择性β1-AR阻滞剂阿替洛尔,对12例增生期血管瘤组织和10例退化期血管瘤组织的β-AR进行双位点竞争结合实验,并根据双位点数学模型,测算出增生期血管瘤和退化期血管瘤组织中的β1-AR及β2-AR的密度和亲和力(抑制常数)。结果增生期血管瘤和退化期血管瘤组织中均存在高亲和力的β1-AR和低亲和力的β2-AR。增生期血管瘤组织中β1-AR的亲和力(0.26±0.09nmol/L)和β2-AR的亲和力(2.94±0.31nmol/L)分别与退化期相比(0.27±0.06nmol/L,2.91±0.53nmol/L),差异均无统计学意义(均P0.05)。增生期血管瘤组织中β1-AR的密度(47.31±7.76 fmol/mg protein)和β2-AR的密度(172.21±30.56fmol/mg protein)分别高于退化期(24.09±5.17fmol/mg protein,73.48±13.34fmol/mg protein),且均有统计学意义(均P0.01)。结论血管瘤组织中存在β1-AR和β2-AR,且以β2-AR为主;增生期血管瘤组织中β1-AR和β2-AR的密度明显上调而亲和力无明显变化;血管瘤的增生可能与其β1-AR和β2-AR尤其是β2-AR的密度上调有关。
[Abstract]:Objective to study the density and affinity of 尾-adrenergic receptor (尾-ARR) in hemangioma and its significance in the pathogenesis of hemangioma. Methods double site competitive binding tests were performed in 12 hyperplastic hemangioma tissues and 10 degenerative hemangioma tissues by radiolabelling non-selective 尾 -AR blocker 125I- indolol and selective 尾 1-AR blocker atenolol. The density and affinity (inhibition constant) of 尾 1-AR and 尾 2-AR in proliferative and degenerative hemangioma tissues were calculated according to the two-locus mathematical model. Results there were high affinity 尾 1-AR and low affinity 尾 2-ARin in proliferative hemangioma and degenerative hemangioma. The affinities of 尾 1-AR and 尾 2-AR in proliferative hemangioma tissues were 0.26 卤0.09 nmol / L and 2.94 卤0.31 nmol / L, respectively, which were not significantly different from those in degenerative phase (0.27 卤0.06 nmol / L, 2.91 卤0.53 nmol / L). The density of 尾 1-AR and 尾 2-AR in proliferative hemangioma was 47.31 卤7.76 fmol/mg and 172.21 卤30.56fmol/mg, respectively, which was significantly higher than that in degenerative phase (24.09 卤5.17fmol/mg protein 73.48 卤13.34fmol/mg) (all P 0.01). Conclusion there are 尾 1-AR and 尾 2-ARs in hemangioma tissues, mainly 尾 2-AR, the density of 尾 1-AR and 尾 2-AR in proliferative hemangioma tissues is obviously up-regulated, and the affinity of 尾 1-AR and 尾 2-AR is not changed obviously, and the proliferation of hemangioma may be related to the up-regulation of 尾 1-AR and 尾 2-AR, especially 尾 2-AR density.
【作者单位】：深圳市儿童医院皮肤科;西安交通大学第二医院皮肤科;
【基金】：广东省医学科研基金项目(A2011560)
【分类号】：R739.5

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