人类疱疹病毒8型K1和K15亚型与鳞癌的相关性研究

发布时间：2018-05-09 22:14

本文选题：皮肤鳞癌 + 食管鳞癌　；参考：《石河子大学》2011年硕士论文

【摘要】：目的：确定皮肤鳞癌和食管鳞癌中感染HHV-8的阳性率,比较两种鳞癌间感染HHV-8的差异性,确定感染HHV-8 K1基因亚型及K15等位基因型。并初步探讨感染人类疱疹病毒8型K1、K15与鳞状细胞癌的发病关系。方法：对40例皮肤鳞癌、40例食管鳞癌石蜡包埋组织使用序列特异性引物-巢式PCR技术(NestedPCR)对人类疱疹病毒8型的DNA抽提,对K1和K15基因的部分片段进行扩增、双向测序,使用DNASTAR软件、Crustal W软件和PHYLIP软件包对测序结果进行系统发生学分析,从而确定HHV-8K1和K15基因亚型,最后运用χ2检验和Fisher确切概率法对结果进行统计学分析。结果：(K1检测结果)：①40例皮肤鳞癌9例(22.5%)感染HHV-8 K1,40例食管鳞癌8例(20%)感染HHV-8 K1,HHV-8在皮肤鳞癌与食管鳞癌感染率组间比较差异无统计学意义(P0.05),即80例鳞癌中有17例感染HHV-8,这17例病毒株中,14例是K1-C亚型占82.35%(均为C2),3例是K1-A亚型(2例为A3,1例为A1)；17例均为K15-P等位基因型占100%；14例为C/P嵌合型(其中14例为C2/P嵌合型)占82.35%。新疆鳞癌感染的HHV-8主要属于K1-C亚型和K15-P等位基因型,以C2/P嵌合型为主,同时也存在部分的K1-A亚型感染者。②皮肤鳞癌感染HHV-8 K1亚型1例A亚型,8例C亚型,食管鳞癌感染HHV-8 K1亚型2例A亚型,5例C亚型,未发现其他亚型。(K15检测结果)①9例皮肤鳞癌(22.5%)石蜡包埋组织中检测为HHV-8 K15P型,8例食管鳞癌(20%)石蜡包埋组织中检测到HHV-8K15P型,两种肿瘤均未检测出K15M基因型；②HHV-8在皮肤鳞癌与食管鳞癌感染率组间比较差异无统计学意义(P0.05)。结论：①新疆鳞癌与新疆不同民族自然人群血清中HHV-8检出率相近,提示HHV-8在鳞状细胞癌中的感染或许一种随机感染,与鳞状细胞癌的发病并没有直接的联系。②感染皮肤鳞癌与食管鳞癌感染HHV-8在组间比较差异无统计学意义。③鳞状细胞癌中感染的HHV-8亚型主要为K1-C亚型和K15 P等位基因型,存在部分的K1-A亚型。
[Abstract]:Objective: to determine the positive rate of HHV-8 infection in squamous cell carcinoma of skin and esophagus, to compare the difference of HHV-8 infection between the two types of squamous cell carcinoma, and to determine the subtype of HHV-8 K1 gene and the genotype of K15 allele. To investigate the relationship between human herpesvirus 8 K1 K15 infection and squamous cell carcinoma (SCC). Methods: DNA of human herpesvirus 8 was extracted from 40 cases of squamous cell carcinoma of skin and 40 cases of esophageal squamous cell carcinoma with paraffin embedded tissue using sequence specific primer nested PCR technique. The partial fragments of K1 and K15 genes were amplified and sequenced. DNASTAR software Crustal W software and PHYLIP software package were used to analyze the sequence results, and the subtypes of HHV-8K1 and K15 genes were determined. Finally, the results were statistically analyzed by 蠂 2 test and Fisher exact probability method. Results there was no significant difference in the infection rate of HHV-8 K1 and esophageal squamous cell carcinoma between HHV-8 K1 and esophageal squamous cell carcinoma group (P 0.05), that is, 17 out of 80 cases of squamous cell carcinoma (17 cases of squamous cell carcinoma of the esophagus) were infected with HHV-8 K1 and HHV-8 (P < 0.05). The results showed that there was no significant difference between the infection rate of HHV-8 K1 and esophageal squamous cell carcinoma (P 0.05), that is, 17 out of 80 cases of squamous cell carcinoma of the esophagus (17 cases of squamous carcinoma of the esophagus) Of the 17 cases infected with HHV-8, 14 cases were K1-C subtype (3 cases were K1-A subtype), 2 cases were A3, 1 case was A3, 17 cases were K15-P allele, 14 cases were C- P chimeric type (14 cases were C2P chimeric type), accounting for 82.35%. The HHV-8 of squamous cell carcinoma infection in Xinjiang mainly belonged to K1-C subtype and K15-P allele, and C2 / P chimeric type was the main genotype. There were also some K1-A subtype infected with HHV-8 K1 subtype A subtype and 8 cases with C subtype of HHV-8 K1 subtype. HHV-8 K1 subtype A subtype was detected in 2 cases of esophageal squamous cell carcinoma infection. No other subtypes were found. K15 was detected in 19 cases of squamous cell carcinoma of skin (22. 5%) HHV-8K15P type was detected in paraffin-embedded tissues of 8 cases of HHV-8 K15P type and 8 cases of esophageal squamous cell carcinoma. There was no significant difference of K15M genotype 2HHV-8 in the infection rate of cutaneous squamous cell carcinoma and esophageal squamous cell carcinoma between the two groups (P 0.05). Conclusion the detection rate of HHV-8 in serum of Xinjiang squamous cell carcinoma is similar to that of different ethnic groups in Xinjiang, suggesting that the infection of HHV-8 in squamous cell carcinoma may be a random infection. There was no direct relationship between squamous cell carcinoma (SCC) and squamous cell carcinoma (SCC). 2 there was no significant difference between the infected SCC and esophageal squamous cell carcinoma (HHV-8) infection. The HHV-8 subtypes of SCC infection were mainly K1-C subtype and K15 P allele. There are partial K1-A subtypes.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R735.1;R739.5

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