神经鞘氨醇磷酸胆碱对人真皮成纤维细胞表达MMP-1,MMP-3和TIMP-1的影响

发布时间：2018-05-22 07:26

本文选题：神经鞘氨醇磷酸胆碱 + 成纤维细胞　；参考：《中国皮肤性病学杂志》2017年02期

【摘要】：目的研究神经鞘氨醇磷酸胆碱(shingosylphosphorylcholine,SPC)对体外培养人真皮成纤维细胞分泌基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-3(MMP-3)、基质金属蛋白酶内源性组织抑制剂-1(TIMP-1)的影响,探索在促进皮肤创伤愈合方面SPC的作用机制。方法通过MTT的方法检测SPC在成纤维细胞增殖效率方面的作用。利用RT-PCR和Western blotting的方法检测体外培养人真皮成纤维细胞经5μmol/L SPC诱导后,表达损伤修复相关蛋白MMP-1,MMP-3,TIMP-1的情况。结果一定浓度的SPC具有促进成纤维细胞增殖的作用。5μmol/L SPC具有上调成纤维细胞表达MMP-1,MMP-3,TIMP-1 m RNA和蛋白的作用(P0.05)。结论实验证实体外培养人真皮成纤维细胞在经SPC干预后,其表达细胞外基质调节蛋白MMP-1,MMP-3,TIMP-1的作用得到增强,提示SPC具有促进皮肤创伤愈合的作用。
[Abstract]:Objective to study the effect of SPCs on the secretion of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP)-3 and matrix metalloproteinase (MMP)-1 (TIMP-1) in human dermal fibroblasts in vitro, and to investigate the effects of SPCs on the secretion of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP-2) and matrix metalloproteinase inhibitor (MMP-1) in vitro. To explore the mechanism of SPC in promoting skin wound healing. Methods the effect of SPC on the proliferation of fibroblasts was detected by MTT. RT-PCR and Western blotting were used to detect the expression of MMP-1, MMP-3 and TIMP-1 in cultured human dermal fibroblasts induced by 5 渭 mol/L SPC. Results A certain concentration of SPC could promote the proliferation of fibroblasts. 5 渭 mol/L SPC could up-regulate the expression of MMP-1, MMP-3, TIMP-1 RNA and protein in fibroblasts. Conclusion the results suggest that the expression of MMP-1, MMP-3 and TIMP-1 in cultured human dermal fibroblasts is enhanced after the intervention of SPC, suggesting that SPC can promote the healing of skin trauma.
【作者单位】：大连市第三人民医院皮肤科;大连医科大学附属第一医院皮肤科;
【分类号】：R751

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