人乳头瘤病毒所致皮肤疣的病毒检测与分型

发布时间：2018-05-25 05:28

本文选题：人乳头瘤病毒 + 寻常疣　；参考：《吉林大学》2012年硕士论文

【摘要】：背景：人乳头瘤病毒（human papillomavirus HPV）可引起皮肤和粘膜良性增生，导致各种皮肤疣的发生，可引起生殖器皮肤黏膜恶性疾病。不同HPV亚型所引起的疾病种类也各不相同。而且HPV感染型别有种族和地域差异性。目的：研究本地患者HPV感染皮损标本中HPV亚型的分布。分析，评价聚合酶链反应（PCR）-反向点杂交法(reverse dot blot)检测HPV亚型的灵敏度、准确性和临床应用价值，为下一步建立HPV感染动物模型，进行药物筛选工作打下基础。方法：采用PCR-反向点杂交法对2011年10月至2012年4月在我院皮肤科就诊的40例寻常疣和尖锐湿疣标本进行HPV DNA的基因分型检测。其中15例寻常疣标本，25例尖锐湿疣标本。年龄12-70岁。结果：在15例寻常疣标本中，HPV的检出例数为11例，检出率为73.3%。检测出HPV型别分别为：HPV1型7例，占总检出例数的63.6%；HPV2型2例，占18.2%，HPV1，，2型混合感染2例，占18.2%。在25例尖锐湿疣标本中，HPV的检出例数为23例，检出率为92%。检测出HPV型别分别为：单一型感染14例，占60.9%，其中HPV6型5例，占21.7%；HPV11型9例，占39.1%。混合感染9例，占39.1%。其中HPV6,11型混合感染3例，占13%；HPV6，16型混合感染2例，占8.7%；HPV6，18型2例，占8.7%；HPV11，52型1例，占4.3%；HPV11，53型1例，占4.3%。结论：共检出2种HPV亚型与寻常疣有关，6种亚型与尖锐湿疣有关。寻常疣中HPV1型感染率最高，达63.6%；尖锐湿疣中HPV11型感染率最高，达39.1%。我们还发现了尖锐湿疣中存在HPV16，18，52，53四种高危型别混合感染。结果显示，PCR-反向点杂交法检测皮肤疣标本中HPV亚型敏感性高，检出率高，适合临床应用。
[Abstract]:Background: Human papillomavirus (HPV) can cause benign hyperplasia of skin and mucosa, lead to the occurrence of various skin warts and lead to malignant diseases of genital skin and mucosa. The types of diseases caused by different HPV subtypes are also different. And there are ethnic and regional differences in the type of HPV infection. Objective: To study the distribution of HPV subtypes in skin lesions of local patients with HPV infection. To evaluate the sensitivity, accuracy and clinical value of polymerase chain reaction-reverse dot hybridization (PCR) for detecting HPV subtypes, and to lay a foundation for the establishment of animal model of HPV infection and drug screening. Methods: HPV DNA genotyping of 40 specimens of verruca vulgaris and condyloma acuminatum from October 2011 to April 2012 was detected by PCR- reverse dot hybridization. 15 cases of verruca vulgaris were collected from 25 cases of condyloma acuminatum. Age 12-70. Results: Among 15 specimens of verruca vulgaris, 11 cases were detected and the detection rate was 73.3%. There were 7 cases of HPV type 1, accounting for 63.6 cases of HPV2 type, and 2 cases of mixed infection of HPV1 type 2 cases (18.2%). In 25 cases of condyloma acuminatum, 23 cases were detected and the detection rate was 92%. There were 14 cases of single type infection (60.9%), 5 cases of HPV6 type, 9 cases of HPV11 type (39.1%). Mixed infection was found in 9 cases (39.1%). Among them, there were 3 cases of mixed infection of HPV6 / 11 type, 2 cases of mixed infection of HPV6 / 16 type, 2 cases of HPV618 type, 1 case of HPV1152 type, 1 case of HPV1153 type, and 1 case of HPV1153 type. Conclusion: Two HPV subtypes were found to be associated with verruca vulgaris and 6 subtypes were associated with condyloma acuminatum. The infection rate of HPV1 type was the highest in verruca vulgaris (63.6%), and the infection rate of HPV11 type in condyloma acuminatum was the highest (39.1%). We also found four high risk types of mixed infection in condyloma acuminatum. The results showed that PCR- reverse dot hybridization was suitable for clinical application in the detection of HPV subtypes in patients with verruca skin.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R752.5

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