凋亡相关蛋白Livin和Caspase-3在日光性角化病发病机制中的作用研究

发布时间：2018-06-05 17:27

本文选题：日光性角化病 + 皮肤鳞状细胞癌　；参考：《泸州医学院学报》2015年05期

【摘要】：目的:探讨凋亡相关蛋白Livin和Caspase-3在皮肤癌前期病变—日光性角化病发病机制中的作用及其相互关系。方法:应用免疫组织化学方法检测36例日光性角化病组织、16例正常人曝光部位和18例正常人非曝光部位皮肤组织和30例皮肤鳞状细胞癌中Livin和Caspase-3表达情况。结果:Livin蛋白在免疫组织化学中阳性着色位于细胞胞浆,其日光性角化病组、皮肤鳞癌组、正常曝光组和正常非曝光组的阳性表达率分别为61.11%、86.67%、12.50%和11.11%。Caspase-3蛋白免疫组织化学中阳性着色位于细胞胞浆,其日光性角化病组、皮肤鳞癌组、正常曝光组和正常非曝光组的阳性表达率分别为52.78%、36.67%、87.50%和83.33%。结论:在正常皮肤组织、日光性角化病、皮肤鳞状细胞癌中Livin的表达率及表达强度呈现增高趋势,Caspase-3的表达率及表达强度呈现降低趋势,统计学分析提示二者在各组样本之间的表达存在负相关性。
[Abstract]:Aim: to investigate the role and relationship of apoptosis-related proteins Livin and Caspase-3 in the pathogenesis of skin precancerous lesions-sunlight keratosis. Methods: the expression of Livin and Caspase-3 in skin tissues of 36 cases of sunburn keratosis and 18 cases of unexposed skin tissues and 30 cases of squamous cell carcinoma of skin were detected by immunohistochemical method. Results the positive staining of the protein was located in the cytoplasm, the sunburn keratosis group and the skin squamous cell carcinoma group. The positive expression rates of normal exposure group and normal non-exposure group were 61.1166.67% and 12.50%, respectively. The positive staining of 11.11%.Caspase-3 protein in cytoplasm was found in the cytoplasm of normal exposure group and normal non-exposure group. The positive expression rates of normal exposure group and normal non-exposure group were 52.78% and 36.67%, 87.50% and 83.33%, respectively. Conclusion: the expression rate and intensity of Livin in normal skin tissue, sunburn keratosis and squamous cell carcinoma of skin showed an increasing trend. The expression rate and intensity of Caspase-3 showed a decreasing trend. Statistical analysis showed that there was a negative correlation between the two groups.
【作者单位】：四川医科大学附属第一医院皮肤科;
【基金】：泸州市科技局项目(2012-S-37)
【分类号】：R758.1

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