DNMT1在不同年龄组人群角质形成细胞中的表达及意义

发布时间：2018-06-19 04:13

本文选题：DNMT + 表皮衰老　；参考：《重庆医科大学学报》2016年10期

【摘要】：目的:检测不同年龄组非曝光部位表皮组织的角质形成细胞中DNA甲基化转移酶1(DNA methyltransferase 1,DNMT1)蛋白和m RNA的表达水平,初步探讨DNMT1在人表皮年龄衰老过程中的意义。方法:收集40例手术患者非曝光部位的表皮标本,根据年龄分为4组:儿童组(0~14岁)、青年组(15~39岁)、中年组(40~59岁)和老年组(60岁及以上),用免疫组织化学、RTPCR及Western blot检测每例表皮组织中DNMT1蛋白和DNMT1 m RNA表达量,分析不同年龄组之间DNMT1表达水平的差异。结果:DNMT1主要表达于各组人表皮角质形成细胞的细胞核内,角质形成细胞DNMT1蛋白及m RNA表达依次在儿童组、青年组、中年组和老年组中呈下降趋势。儿童组、青年组、中年组和老年组角质形成细胞DNMT1阳性表达率分别为84.7%、69.4%、51.1%、12.3%,表达率得分分别为:23.10分、19.30分、14.40分和3.80分,采用LSD检验法对4组间进行两两比较,表明角质形成细胞DNMT1阳性表达率随年龄的增长呈递减趋势(均P0.05)。DNMT1 m RNA相对表达量为1.347、0.817、0.567和0.202,DNMT1蛋白的相对表达量为1.102、0.494、0.320和0.088,统计学分析DNMT1随着年龄的增长表达量降低(两两对比,均P0.05)。结论:随着年龄的增长,DNMT1的表达水平下降,推测人表皮衰老可能与DNMT1的表达下调有一定的关联。
[Abstract]:Aim: to investigate the expression of DNMT1 protein and mRNA in keratinocytes of unexposed epidermis of different age groups, and to explore the significance of DNMT1 in the aging of human epidermis. Methods: 40 unexposed epidermis specimens were collected from 40 patients. According to their age, they were divided into four groups: the children group (14 years old), the young group (1539 years old) and the middle-aged group (4059 years old) and the elderly group (60 years old and above). The expression of DNMT1 protein and DNMT1 mRNA in each epidermis was detected by immunohistochemistry RT-PCR and Western blot. The difference of DNMT1 expression between different age groups was analyzed. Results DNMT1 was mainly expressed in the nuclei of human epidermal keratinocytes in each group. The expression of DNMT1 protein and mRNA in keratinocytes was decreased in the children group, the young group, the middle age group and the old group. The positive rate of DNMT1 expression in children group, youth group, middle age group and elderly group was 84.7and 69.40.51.1% and 12.33min, respectively. The expression rates were 23.10, 19.30, 14.40 and 3.80, respectively. The four groups were compared with each other by LSD test. The results showed that the positive expression rate of DNMT1 in keratinocytes decreased with age (the relative expression levels of DNMT1 mRNA were 1.347U 0.8170.67 and 0.202 DNMT1, respectively) were 1.102 and 0.4940.320 and 0.088, respectively. The expression of DNMT1 decreased with age (P < 0.05). Conclusion: the expression level of DNMT1 decreases with age, suggesting that human epidermal senescence may be related to the down-regulation of DNMT1 expression.
【作者单位】：重庆医科大学附属第一医院整形外科;
【分类号】：R751

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