卤米松与他扎罗汀对角质形成细胞RAR-γ和RXR-α表达的影响

发布时间：2018-06-20 19:58

本文选题：RNA + 信使　；参考：《重庆医学》2016年15期

【摘要】：目的观察不同浓度卤米松和他扎罗汀对体外培养人永生化HaCaT角质形成细胞的维甲酸受体(RAR)-γ和维甲酸X受体(RXR)-α表达的影响,探讨卤米松对他扎罗汀治疗银屑病效应的潜在影响。方法 CCK-8方法检测不同浓度卤米松和他扎罗汀对HaCaT细胞增殖的影响;Western blot法和实时荧光定量PCR(RT-PCR)法检测不同浓度卤米松和他扎罗汀作用12h、24h后,HaCaT细胞中RAR-γ和RXR-α的mRNA水平和蛋白水平的变化。结果卤米松和他扎罗汀均可抑制角质形成细胞增殖。卤米松使HaCaT细胞的RAR-γ和RXR-αmRNA水平和蛋白水平升高。他扎罗汀作用后RAR-γ和RXR-α的mRNA水平降低,而蛋白水平在12h升高,24h降低。结论卤米松作用角质形成细胞后可上调RAR-γ和RXR-α表达,这可能有助于提高角质形成细胞对他扎罗汀作用的反应性,从而提高他扎罗汀的作用效应。
[Abstract]:Objective to observe the effects of halomethasone and tazarotene on the expression of retinoic acid receptor (RRA)-纬 and retinoic acid X receptor (RXR- 伪) in cultured human immortalized HaCaT keratinocytes in vitro, and to explore the potential effects of halomethasone on the treatment of psoriasis by tazarotene. Methods the effects of different concentrations of halomethasone and tazarotene on the proliferation of HaCaT cells were detected by CCK-8 method. The mRNA levels of RAR- 纬 and RXR- 伪 in HaCaT cells were detected by Western blot assay and real-time fluorescence quantitative PCR- PCR- PCRmethod. And changes in protein levels. Results both halomethasone and tazarotene could inhibit the proliferation of keratinocytes. Halomethasone increased RAR- 纬 and RXR- 伪 mRNA and protein levels in HaCaT cells. The mRNA levels of RAR- 纬 and RXR- 伪 decreased, while the protein levels increased at 12 h and decreased 24 h after treatment with tazarotene. Conclusion halomethasone can up-regulate the expression of RAR- 纬 and RXR- 伪 in keratinocytes, which may be helpful to enhance the responsiveness of keratinocytes to the action of tacarotene, thereby enhancing the effect of tacarotene.
【作者单位】：第三军医大学新桥医院皮肤科;
【基金】：中华医学会皮肤性病学分会-澳美制药银屑病研究基金资助项目
【分类号】：R758.63

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