梅毒螺旋体外膜脂蛋白TP15、TP17、TP47在梅毒免疫中的作用

发布时间：2018-06-25 17:29

本文选题：梅毒 + TP15　；参考：《山西医科大学》2010年硕士论文

【摘要】： 目的比较性研究一期,二期、潜伏梅毒及血清固定型梅毒患者对梅毒螺旋体外膜脂蛋白TP15、TP17、TP47抗原特异性体液免疫和细胞免疫反应。了解梅毒螺旋体外膜脂蛋白TP15,、TP17、TP47抗原在梅毒特异性细胞免疫和体液免疫中的作用。方法采集正常对照者、一期梅毒、二期梅毒、潜伏梅毒患者以及治疗后两年RPR未转阴血清固定型梅毒患者的外周血,分离血清和外周血单个核细胞,将梅毒螺旋体TP15、TP17、TP47蛋白作为特异性梅毒抗原,采用immuno-slot blot method方法检测梅毒患者血清中TP15、TP17和TP47特异性抗体状况。通过酶联免疫斑点技术(enzyme-linked immuno-spot, Elispot)检测梅毒患者中TP15、TP17和TP47刺激细胞产生细胞因子(INF-γ)。结果本试验通过immun-solt bolt方法对8例一期梅毒,15例二期梅毒,15例潜伏梅毒和13例血清固定型梅毒患者TP15、TP17和TP47进行检测,结果：一期梅毒中TP15的阳性率约为25%,TP17为100%,TP47为87.5%。TP15与TP17间χ2=4.17,P0.05,有统计学意义；TP15与TP47间χ2=4.0,P0.05,存在统计学差异；TP17与TP47之间χ2=0.00,P0.05,差异无显著性；二期梅毒中,TP15阳性率为80%,TP17为100%,TP15与TP17和TP47间χ2=1.75,P0.05,无统计学差异。TP47的阳性率的检出率也很高,约为93.4%。各组别间阳性率比较较高的为二期梅毒。本研究利用ELISPOT技术检测三种梅毒外膜脂蛋白,结果：与正常对照组相比,二期梅毒各抗原刺激的差异均有显著性,一期、二期和血清固定型梅毒针对TP17抗原产生的细胞因子INF-γ与正常对照组相比存在统计学差异,潜伏梅毒对三种抗原产生的INF-γ与正常对照组相比均无统计学差异。结论用TP15、TP17、TP47三种重组蛋白作为抗原刺激物通过,Immun-solt blod技术对各期梅毒进行体液免疫检测,Elispot技术进行细胞免疫检测,发现这三种抗原可引起广泛的特异性体液免疫,而特异性细胞免疫反应相对较弱。为梅毒患者在发病中的免疫变化情况提供实验室依据,为探讨梅毒的发病机制提供新的思路。
[Abstract]:Objective to compare the specific humoral and cellular immune responses of patients with latent syphilis and serum-fixed syphilis to TP15 TP17 TP47 antigen of Treponema pallidum. Objective: to investigate the role of TP17 / TP47 antigen of Treponema pallidum outer membrane lipoprotein TP15 in specific cellular and humoral immunity of syphilis. Methods Peripheral blood samples were collected from normal controls, primary syphilis, secondary syphilis, latent syphilis, and two years after treatment with RPR non-negative serum-fixed syphilis, and the serum and peripheral blood mononuclear cells were isolated. Treponema pallidum TP15, TP17, TP47 were used as specific syphilis antigens, and the specific antibodies of TP15, TP17 and TP47 in serum of syphilis patients were detected by immuno-slot blot method. TP15, TP17 and TP47 stimulated cytokines (INF- 纬) were detected by enzyme-linked immuno spot (Elispot) in patients with syphilis. Results TP15, TP17 and TP47 were detected by immun-solt bolt in 8 cases of primary syphilis and 15 cases of secondary syphilis and 13 cases of serum fixed syphilis. Results: the positive rate of TP15 in primary syphilis was about 25%. TP17 was 100 and TP47 was 87.5.TP15 and TP17 were 87.5.TP15 and TP17 蠂 24.17g / P0.05. there was statistical significance between TP15 and TP47 (蠂 24.0 P0.05), there was no significant difference between TP17 and TP47 (蠂 20.00P 0.05). In secondary syphilis, the positive rate of TP15 was 80% and TP17 was 100%. There was no statistical difference between TP15 and TP17 and TP47. The positive rate of TP15 was also very high, about 93.4. The higher positive rate of syphilis in each group was secondary syphilis. In this study, three kinds of syphilis outer membrane lipoproteins were detected by Elispot technique. Results: compared with the normal control group, there were significant differences in each antigen stimulation of secondary syphilis. The levels of cytokine INF- 纬 against TP17 antigen in secondary and serum stationary syphilis were significantly different from those in normal control group, but there was no significant difference between latent syphilis and normal control group. Conclusion the three recombinant proteins of TP15, TP17 and TP47 were used as antigenic stimulators to detect the cellular immunity of syphilis by immuno-immunoassay with Immun-solt blod. It was found that these three antigens could induce a wide range of specific humoral immunity. The specific cellular immune response was relatively weak. To provide laboratory basis for the immune changes of syphilis patients, and to provide a new way to explore the pathogenesis of syphilis.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R759.1

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