Toll样受体2、4基因多态性与麻风的相关性研究

发布时间：2018-06-29 22:31

本文选题：麻风 + Toll样受体2　；参考：《遵义医学院》2010年硕士论文

【摘要】： 目的：研究Toll样受体2(Toll-like receptor2, TLR2) (597T/C)和TLR4(896A/G)基因单核苷酸多态性(SNP)与麻风的相关性,探讨麻风的遗传易感性。方法：选择中国贵州毕节地区麻风病例117例[按类型分为瘤型(79例)和结核样型(38例)、按集聚性分为家族集聚性(43例)和非家族集聚性(74例)],健康正常对照50例；用聚合酶链反应限制性片断长度多态性(PCR-RFLP)法分析各样本TLR2 597T/C和TLR4 896A/G位点的基因型,比较麻风和正常组基因型分布所占比率以及麻风病例中各组基因型分别占有比率。SPSS16.0软件的x2检验对各组例数进行统计处理。结果：TLR2(597T/C)TT、TC、CC三种基因型在麻风患者和健康人中的分布频率分别为46.2%、40.2%、13.6%和48.0%、42.0%、10.0%,无明显差异(x2=0.431,P0.05)；瘤型和结核样TT、TC、CC基因型所占比率分别为44.3%、40.5%、15.2%和50.0%、39.5%、10.5%,差异无统计学意义(x2=0.595,P0.05)；家族集聚性和非家族性TT、TC、CC基因型所占比率分别为48.8%、37.2%、14.0%和44.6%、41.9%、13.5%,差异无统计学意义(x2=0.258,P0.05))。所有研究对象未检测到TLR4(896A/G)多态性,基因型均为896AA野生纯合子。结论：TLR2(597T/C)、TLR4(896T/G)多态性与中国贵州毕节地区麻风无相关,TLR4(896A/G)多态性在中国人中罕见。
[Abstract]:Objective: to study the association between Toll-like receptor 2 (TLR2) (597T / C) and TLR4 (896A / G) gene single nucleotide polymorphism (SNP) in leprosy and to explore the genetic susceptibility of leprosy. Methods: 117 cases of leprosy in Bijie District, Guizhou Province, China were divided into tumor type (79 cases) and tuberculoid type (38 cases), familial agglomeration (43 cases) and non-familial agglomeration (74 cases), and healthy control group (50 cases). The genotypes of TLR2 597T / C and TLR4896A / G loci were analyzed by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The percentage of genotype distribution in leprosy group and normal group and the percentage of genotype in each group were compared. SPSS 16.0 software x2 test was used to analyze the number of cases in each group. Results the distribution frequency of TLR2 (597T / C) TCCC genotypes in leprosy patients and healthy persons was 46.2% and 48.2%, respectively, and there was no significant difference between them (x20.431P0.05), and the percentage of TCCCC genotypes in tumor-type and tuberculoid TTTCnC was 44.3%, 40.515.2% and 59.55.50%, respectively, and there was no significant difference between them (x20.595P0.05). The percentage of TCC-CC genotypes was 48.8% and 44.69%, respectively. There was no significant difference between them (x20.258%, P0.05). No TLR4 (896A / G) polymorphism was detected in all subjects and the genotype was 896AA wild homozygote. ConclusionTwo TLR2 (597T / C) TLR4 (896T / G) polymorphism is not associated with leprosy in Bijie region, Guizhou Province, China. The polymorphism of TLR4 (896A / G) is rare in Chinese.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R755

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