藏雪莲多糖减轻UVB辐射后HaCaT细胞氧化损伤的实验研究

发布时间：2018-06-30 00:16

本文选题：紫外线辐射 + 人永生化角质细胞　；参考：《青海大学》2017年硕士论文

【摘要】：目的研究藏雪莲植物多糖是否能对体外UVB(Ultraviolet b,中波紫外线)诱导的人永生化角质形成细胞(Ha Ca T细胞)的氧化损伤起保护作用。方法应用体外培养的细胞实验模型培养人永生化表皮(Ha Ca T细胞),按照辐射能量不同,分为高辐射组,低辐射组。应用提纯后不同浓度的藏雪莲多糖对辐射细胞进行干预,分为对照组(正常培养,无辐射,无多糖)、辐射组(辐射,无多糖)、低浓度臧雪莲多糖组、高浓度臧雪莲多糖组。多糖提前1h添加。照射后各组细胞培养18h。应用台盼蓝染色法对辐射后各组细胞形态与凋亡状况观察并摄片,应用MTS法测细胞增殖;抗氧化酶测定:酶标法测GSH(谷胱甘肽)含量,TBA法测定MDA(细胞丙二醛)含量,可见光法测定CAT(过氧化氢酶)及WST-1法测定SOD(超氧化物歧化酶)活力。结果与辐射组相比,给予不同浓度的藏雪莲多糖后,蓝染数下降,细胞结构完整性上升,减少细胞的死亡率;增殖活性提高;降低MDA含量,使GSH含量、SOD及CAT活性上升,与辐射组比较均具有统计学差异(p0.05)。结论经过不同能量UVB辐射后的Ha Ca T细胞在给予一定剂量的藏雪莲多糖形后,可有效减轻光损伤造成的细胞结构破坏,减少细胞凋亡。
[Abstract]:Objective to study whether the polysaccharides of Saussurea lanceolata can protect human immortalized keratinocytes (Ha Ca T cells) from oxidative damage induced by UVB (Ultraviolet B) in vitro. Methods Human immortalized epidermis (Ha Ca T cells) were cultured in vitro and divided into high radiation group and low radiation group according to different radiation energy. After purification, different concentrations of Tibetan snow lotus polysaccharides were used to interfere with radiation cells and were divided into control group (normal culture, no radiation, no polysaccharide), radiation group (radiation, no polysaccharide), low concentration Zang Xuelian polysaccharide group and high concentration Zang Xuelian polysaccharide group. Polysaccharide was added 1 hour earlier. The cells were cultured for 18 hours after irradiation. Cell morphology and apoptosis were observed by trypan blue staining, cell proliferation was measured by MTS method, GSH (glutathione) content was measured by enzyme labeling method and MDA (cell malondialdehyde) content was measured by TBA method. Cat (catalase) and SOD (superoxide dismutase) activity were measured by visible light method and WST-1 method respectively. Results compared with radiation group, the blue staining number decreased, cell structural integrity increased, cell death rate decreased, proliferation activity increased, MDA content decreased, and GSH content SOD and cat activities increased, compared with radiation group. There was statistical difference between the two groups (p0.05). Conclusion the Ha-Ca T cells irradiated with different energy UVB can effectively reduce the damage of cell structure and apoptosis caused by light damage after given a certain dose of polysaccharide form of Saussurea sorbifolia.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R751

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